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Looking at this AB to detect Collagen 7 using immunofluorescence. Does this AB detect the internal alpha chain or the Non collagenous component? I also note that you sell other AB's specific to the internal region and the NC region. I am specifically looking for an AB to type VII that will register the internal region using IF. Appreciate your response!
Asked on Nov 28 2012
Thank you for your inquiry.
I heard back from the lab that our anti-Collagen VII antibody is specific only for helical domains (collagen VII fragments). The non-collagen fragments obtained after pepsin digestion are broken and cannot be detected.
I'm not sure if any of our Collagen VII antibodies will suit your needs, but take a look at ab80790 as this one has quite a few references and has been used for cell staining.
I hope this information helps. Please contact us with any other questions.
Answered on Nov 28 2012