Product nameAnti-Collagen VII antibody [LH7.2]
See all Collagen VII primary antibodies
DescriptionMouse monoclonal [LH7.2] to Collagen VII
Tested applicationsSuitable for: WB, ELISA, IHC-Fr, ICC/IF, ICC, Dot blot, IHC-Pmore details
Species reactivityReacts with: Sheep, Goat, Horse, Guinea pig, Cow, Human
Full length native protein (purified) corresponding to Human Collagen VII.
EpitopeRecognizes an epitope located on collagenase digested Type VII collagen (150kD), i.e. the non-helical carboxy terminal region of the Type VII collagen dimer. Immunoelectron microscopy shows antibody binding localised at the inferior border of the lamina densa.
This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016 - GR158908. Lot numbers higher than GR158908 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.”
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.4
Preservative: 0.097% Sodium azide
Concentration information loading...
Purification notesPurified from Tissue culture supernatant.
Our Abpromise guarantee covers the use of ab6312 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependant dilution.
ICC: Use at an assay dependant dilution.
IF: 1/1000. (human or other mammalian frozen sections)
IHC-Fr: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionStratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.
Involvement in diseaseEpidermolysis bullosa acquisita (EBA) is an autoimmune acquired blistering skin disease resulting from autoantibodies to type VII collagen.
Epidermolysis bullosa dystrophica, autosomal dominant
Epidermolysis bullosa dystrophica, autosomal recessive
Epidermolysis bullosa dystrophica, Pasini type
Epidermolysis bullosa dystrophica, Hallopeau-Siemens type
Transient bullous dermolysis of the newborn
Epidermolysis bullosa dystrophica, pretibial type
Epidermolysis bullosa dystrophica, Bart type
Epidermolysis bullosa pruriginosa
Nail disorder, non-syndromic congenital, 8
Epidermolysis bullosa dystrophica, with subcorneal cleavage
Sequence similaritiesContains 1 BPTI/Kunitz inhibitor domain.
Contains 9 fibronectin type-III domains.
Contains 2 VWFA domains.
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
- Information by UniProt
- Alpha 1 type VII collagen antibody
- CO7A1_HUMAN antibody
- Col7a1 antibody
Immunohistochemical staining of Collagen VII in human skin tissue sections with ab6312. Samples were fixed with formaldehyde and enzymatic antigen retrieval was performed with Proteinase K. Tissue was blocked with 1% BSA for 10 minutes at 21°C before ab6312 was added (1/1000) for 2 hours at 21°C. A biotin conjugated goat anti-mouse secondary was used at 1/250.
This product has been referenced in:
- Derr K et al. Fully Three-Dimensional Bioprinted Skin Equivalent Constructs with Validated Morphology and Barrier Function. Tissue Eng Part C Methods 25:334-343 (2019). Read more (PubMed: 31007132) »
- Ponsioen TL et al. Human retinal Müller cells synthesize collagens of the vitreous and vitreoretinal interface in vitro. Mol Vis 14:652-60 (2008). ICC/IF ; Human . Read more (PubMed: 18385800) »