Anti-Collagen VII antibody [LH7.2] (ab6312)

Mouse monoclonal Collagen VII antibody [LH7.2]. Validated in WB, ELISA, IHC, ICC, Dot, ICC/IF and tested in Sheep, Goat, Horse, Guinea pig, Cow, Human. Cited in 1 publication(s).


  • Product name

    Anti-Collagen VII antibody [LH7.2]
    See all Collagen VII primary antibodies
  • Description

    Mouse monoclonal [LH7.2] to Collagen VII
  • Host species

  • Tested applications

    Suitable for: WB, ELISA, IHC-Fr, ICC/IF, ICC, Dot blot, IHC-Pmore details
  • Species reactivity

    Reacts with: Sheep, Goat, Horse, Guinea pig, Cow, Human
  • Immunogen

    Full length native protein (purified) corresponding to Human Collagen VII.

  • Epitope

    Recognizes an epitope located on collagenase digested Type VII collagen (150kD), i.e. the non-helical carboxy terminal region of the Type VII collagen dimer. Immunoelectron microscopy shows antibody binding localised at the inferior border of the lamina densa.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016 - GR158908. Lot numbers higher than GR158908 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.”


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Proprietary Purification
  • Purification notes

    Purified from Tissue culture supernatant.
  • Clonality

  • Clone number

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab6312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot
  • Application notes
    Dot: Use at an assay dependant dilution.
    ELISA: Use at an assay dependant dilution.
    ICC: Use at an assay dependant dilution.
    IF: 1/1000. (human or other mammalian frozen sections)
    IHC-Fr: Use at an assay dependant dilution.
    WB: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.
    • Involvement in disease

      Epidermolysis bullosa acquisita (EBA) is an autoimmune acquired blistering skin disease resulting from autoantibodies to type VII collagen.
      Epidermolysis bullosa dystrophica, autosomal dominant
      Epidermolysis bullosa dystrophica, autosomal recessive
      Epidermolysis bullosa dystrophica, Pasini type
      Epidermolysis bullosa dystrophica, Hallopeau-Siemens type
      Transient bullous dermolysis of the newborn
      Epidermolysis bullosa dystrophica, pretibial type
      Epidermolysis bullosa dystrophica, Bart type
      Epidermolysis bullosa pruriginosa
      Nail disorder, non-syndromic congenital, 8
      Epidermolysis bullosa dystrophica, with subcorneal cleavage
    • Sequence similarities

      Contains 1 BPTI/Kunitz inhibitor domain.
      Contains 9 fibronectin type-III domains.
      Contains 2 VWFA domains.
    • Post-translational

      Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix > basement membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Alpha 1 type VII collagen antibody
      • CO7A1_HUMAN antibody
      • Col7a1 antibody
      • Collagen 7 antibody
      • Collagen alpha 1(VII) chain antibody
      • Collagen alpha-1(VII) chain antibody
      • Collagen type VII alpha 1 antibody
      • Collagen VII alpha 1 polypeptide antibody
      • CollagenVII antibody
      • EBD 1 antibody
      • EBD1 antibody
      • EBDCT antibody
      • EBR 1 antibody
      • EBR1 antibody
      • LC collagen antibody
      • Long chain collagen antibody
      • Long-chain collagen antibody
      see all


    • Immunohistochemical staining of Collagen VII in human skin tissue sections with ab6312. Samples were fixed with formaldehyde and enzymatic antigen retrieval was performed with Proteinase K. Tissue was blocked with 1% BSA for 10 minutes at 21°C before ab6312 was added (1/1000) for 2 hours at 21°C. A biotin conjugated goat anti-mouse secondary was used at 1/250.

      See Abreview


    This product has been referenced in:

    • Derr K  et al. Fully Three-Dimensional Bioprinted Skin Equivalent Constructs with Validated Morphology and Barrier Function. Tissue Eng Part C Methods 25:334-343 (2019). Read more (PubMed: 31007132) »
    • Ponsioen TL  et al. Human retinal Müller cells synthesize collagens of the vitreous and vitreoretinal interface in vitro. Mol Vis 14:652-60 (2008). ICC/IF ; Human . Read more (PubMed: 18385800) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-7 of 7 Abreviews or Q&A

    Immunohistochemistry (Frozen sections)
    Human Tissue sections (Skin)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Jul 11 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: Proteinase K
    Human Tissue sections (Skin)

    Mr. Carl Hobbs

    Verified customer

    Submitted Aug 19 2014


    Thank you for contacting us.
    Both antibodies were run under reducing and denaturing conditions.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Thank you for contacting us.

    I have attached the reference paper which the lab cited as researchers having used this antibody.
    As far as I could see they did do the collagenase treatment and obtained the 150 kDa band. This is most likely the source where the information on the datasheet comes from.

    Let me know if I shall ask the lab additional information in this regard.

    I hope this information is of some help to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!

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    I am very pleased to hear you would like to accept our offer and test ab6312 and ab80790in mouse. This code will give you: 1 free PRIMARY ANTIBODY OR COST OF THE PRODUCT OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview formouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site:

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here:

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    Thank you very much for your enquiry. All the information that we have regarding these antibodies is located on the online datasheets, please refer to them for more information. Here are the expected band sizes for these antibodies: ab7045 - 21.6 kDa ab7778 - Collagen III has a major band at 150kDa under reducing conditions. ab292 - The M.Wt of collagen Type I is at least 95kDa. ab6312 - Recognizes an epitope located on collagenase digested Type VII collagen (150kD), i.e. the non-helical carboxy terminal region of the Type VII collagen dimer. ab6307 - 70 kDa I will get back to you regarding ab6583 and ab6311. Let me know if you have any more questions.

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    The immunogen used to raise this antibody was insoluble fractions prepared from neonatal foreskin epidermal cells. Unfortunately, I have not been able to source a supplier for the recombinant protein.

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