• Product name
    Anti-Collagen VIII alpha 1 antibody
    See all Collagen VIII alpha 1 primary antibodies
  • Description
    Rabbit polyclonal to Collagen VIII alpha 1
  • Host species
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rabbit, Human
  • Immunogen

    A synthetic peptide corresponding to C-terminal residues of human Collagen VIII alpha 1



Our Abpromise guarantee covers the use of ab58776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    ELISA: Use at an assay dependent dilution.
    WB: Use at an assay dependent concentration. Predicted molecular weight: 73 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in artherogenesis.
      Vastatin, the C-terminal fragment comprising the NC1 domain, inhibits aortic endothelial cell proliferation and causes cell apoptosis.
    • Tissue specificity
      Expressed primarily in the subendothelium of large blood vessels. Also expressed in arterioles and venules in muscle, heart, kidney, spleen, umbilical cord, liver and lung and is also found in connective tissue layers around hair follicles, around nerve bundles in muscle, in the dura of the optic nerve, in cornea and sclera, and in the perichondrium of cartilaginous tissues. In the kidney, expressed in mesangial cells, glomerular endothelial cells, and tubular epithelial cells. Also expressed in mast cells, and in astrocytes during the repair process. Expressed in Descemet's membrane. Specifically expressed in peritoneal fibroblasts and mesothelial cells.
    • Sequence similarities
      Contains 1 C1q domain.
    • Post-translational
      Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
      Proteolytically cleaved by neutrophil elastase, in vitro. Proteolytic processing produces the C-terminal NC1 domain fragment, vastatin.
    • Cellular localization
      Secreted > extracellular space > extracellular matrix > basement membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • C3orf7 antibody
      • CO8A1_HUMAN antibody
      • COL8A1 antibody
      • Collagen alpha 1(VIII) chain [Precursor] antibody
      • Endothelial collagen antibody
      • MGC9568 antibody
      • Vastatin antibody
      see all


    This product has been referenced in:
    • Ma ZH  et al. Effect of enhanced expression of COL8A1 on lymphatic metastasis of hepatocellular carcinoma in mice. Exp Ther Med 4:621-626 (2012). WB . Read more (PubMed: 23170115) »
    • Loeffler I  et al. Type VIII Collagen Modulates TGF-{beta}1-induced Proliferation of Mesangial Cells. J Am Soc Nephrol 22:649-63 (2011). WB ; Mouse . Read more (PubMed: 21372207) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Western blot
    Human Cell lysate - whole cell (MDA-MB-231, MCF-7)
    Gel Running Conditions
    Reduced Denaturing (10-20% tris-glycine)
    Loading amount
    35 µg
    MDA-MB-231, MCF-7
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

    Abcam user community

    Verified customer

    Submitted Mar 04 2019


    Yes, I will let you know.

    Thank you so much for your patience and understanding!

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    Thank you for contacting us.

    I have good news: the peptide is available for sale, but is currently backordered for 3 weeks.
    Once I have more information about the catalog number and datasheet I will let you know - hopefully by the end of the week.

    Thank you for your patience.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!

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    Thank you for your inquiry.  A cross-reaction of this antibody with the collagen alpha2 (VIII) chain was not specifically tested. But I can confirm that a cross-reaction is very unlikely because the immunogen sequence only shares 66% homology with alpha2. We expect a possible cross-reaction from 85% homology and more. I am sorry I could not be of more help on this occasion and hope this information was nevertheless helpful. Please do not hesitate to contact me again with any further questions.  

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