Key features and details
- Rabbit polyclonal to Collagen X
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Product nameAnti-Collagen X antibody
See all Collagen X primary antibodies
DescriptionRabbit polyclonal to Collagen X
Specificityab58632 recognizes type X collagen. Exhibits slight cross-reactivity with fibronectin and type II and type IX collagen. Does not cross-react with type I, type III, or type XI collagen.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Full length native protein (purified) Type X collagen from rat chondrosarcoma cells
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: Whole serum
Concentration information loading...
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab58632 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/300.
1/100 - 1/300.
FunctionType X collagen is a product of hyperthrophic chondrotocytes and has been localized to presumptive mineralization zones of hyaline cartilage.
Involvement in diseaseDefects in COL10A1 are the cause of Schmid type metaphyseal chondrodysplasia (SMCD) [MIM:156500]. SMCD is a dominantly inherited disorder of the osseous skeleton. The cardinal features of the phenotype are mild short stature, coxa vara and a waddling gait. Radiography usually shows sclerosis of the ribs, flaring of the metaphyses, and a wide irregular growth plate, especially of the knees. A variant form of SMCD is spondylometaphyseal dysplasia Japanese type. It is characterized by spinal involvement comprising mild platyspondyly, vertebral body abnormalities, and end-plate irregularity.
Sequence similaritiesContains 1 C1q domain.
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- COAA1_HUMAN antibody
- Col10a 1 antibody
- COL10A1 antibody
Anti-Collagen X antibody (ab58632) at 1/500 dilution + HT 1080 (Human fibrosarcoma) Whole Cell Lysate at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 66 + 64 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa, 50 kDa, 98 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 150 seconds
The band observed at 64 kDa could potentially be a cleaved form of Collagen X due to the presence of a 18 amino acid signal peptide.
ab58632 has been referenced in 190 publications.
- Elkhenany HA et al. Bone Marrow Mesenchymal Stem Cell-Derived Tissues are Mechanically Superior to Meniscus Cells. Tissue Eng Part A 27:914-928 (2021). PubMed: 32940137
- Zhang P et al. microRNA-130b downregulation potentiates chondrogenic differentiation of bone marrow mesenchymal stem cells by targeting SOX9. Braz J Med Biol Res 54:e10345 (2021). PubMed: 33624729
- Futrega K et al. Characterisation of ovine bone marrow-derived stromal cells (oBMSC) and evaluation of chondrogenically induced micro-pellets for cartilage tissue repair in vivo. Stem Cell Res Ther 12:26 (2021). PubMed: 33413652
- Serjeant M et al. The Role of Panx3 in Age-Associated and Injury-Induced Intervertebral Disc Degeneration. Int J Mol Sci 22:N/A (2021). PubMed: 33499145
- Lu J et al. Fargesin ameliorates osteoarthritis via macrophage reprogramming by downregulating MAPK and NF-?B pathways. Arthritis Res Ther 23:142 (2021). PubMed: 33990219