Anti-Collagen X antibody (ab58632)

Rabbit polyclonal Collagen X antibody. Validated in WB, IP, IHC, ICC/IF and tested in Mouse, Rat, Rabbit, Cow, Human. Cited in 96 publication(s). Independently reviewed in 9 review(s).

Overview

  • Product name
  • Description
    Rabbit polyclonal to Collagen X
  • Host species
    Rabbit
  • Specificity
    ab58632 recognizes type X collagen. Exhibits slight cross-reactivity with fibronectin and type II and type IX collagen. Does not cross-react with type I, type III, or type XI collagen.
  • Tested applications
    Suitable for: ICC/IF, WB, IP, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Cow, Human
  • Immunogen

    Full length native protein (purified) Type X collagen from rat chondrosarcoma cells

Properties

Applications

Our Abpromise guarantee covers the use of ab58632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/100 - 1/300.
IP 1/20 - 1/40.
IHC-Fr Use at an assay dependent concentration. Enzymatic or heat (41°C) pretreatment is recommended.

Target

  • Function
    Type X collagen is a product of hyperthrophic chondrotocytes and has been localized to presumptive mineralization zones of hyaline cartilage.
  • Involvement in disease
    Defects in COL10A1 are the cause of Schmid type metaphyseal chondrodysplasia (SMCD) [MIM:156500]. SMCD is a dominantly inherited disorder of the osseous skeleton. The cardinal features of the phenotype are mild short stature, coxa vara and a waddling gait. Radiography usually shows sclerosis of the ribs, flaring of the metaphyses, and a wide irregular growth plate, especially of the knees. A variant form of SMCD is spondylometaphyseal dysplasia Japanese type. It is characterized by spinal involvement comprising mild platyspondyly, vertebral body abnormalities, and end-plate irregularity.
  • Sequence similarities
    Contains 1 C1q domain.
  • Post-translational
    modifications
    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • COAA1_HUMAN antibody
    • Col10a 1 antibody
    • COL10A1 antibody
    • Collagen alpha 1(X) chain antibody
    • Collagen alpha-1(X) chain antibody
    • Collagen type X alpha 1 (Schmid metaphyseal chondrodysplasia) antibody
    • Collagen type X alpha 1 antibody
    • Collagen X alpha 1 polypeptide antibody
    • CollagenX antibody
    • fa66d11 antibody
    • fb10c08 antibody
    • OTTHUMP00000040411 antibody
    • Procollagen type X alpha 1 antibody
    • Schmid metaphyseal chondrodysplasia antibody
    • wu:fa66d11 antibody
    • wu:fb10c08 antibody
    see all

Images

  • Immunofluorescence analysis of mouse bone marrow stromal cells, staining Collagen X with ab58632.

    Cells were fixed with formaldehyde, permeabilized with 0.2% Triton X-100 before incubating with primary antibody (1/50) overnight. An AlexaFluor®488-conjugated anti-rabbit IgG (1/500) was used as the secondary antibody.
  • Anti-Collagen X antibody (ab58632) at 1/500 dilution + HT 1080 (Human fibrosarcoma) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 66 + 64 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 37 kDa, 50 kDa, 98 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 150 seconds


    The band observed at 64 kDa could potentially be a cleaved form of Collagen X due to the presence of a 18 amino acid signal peptide.

References

This product has been referenced in:
  • Liao L  et al. Deletion of Runx2 in condylar chondrocytes disrupts TMJ tissue homeostasis. J Cell Physiol 234:3436-3444 (2019). Read more (PubMed: 30387127) »
  • Sun AR  et al. Pro-resolving lipid mediator ameliorates obesity induced osteoarthritis by regulating synovial macrophage polarisation. Sci Rep 9:426 (2019). Read more (PubMed: 30674985) »
See all 109 Publications for this product

Customer reviews and Q&As

1-10 of 18 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mouse growth plate)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric buffer ph6
Permeabilization
No
Specification
mouse growth plate
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 22°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Nov 02 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Cartilage)
Antigen retrieval step
Enzymatic
Permeabilization
Yes - Proteinase K
Specification
Cartilage
Blocking step
4% BSA+ 4% horse serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 12 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Growth plate of tibia)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Antigen Unmasking Solution, Tris-Based
Permeabilization
Yes - Triton X
Specification
Growth plate of tibia
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Dr. Tina Yuan

Verified customer

Submitted Jan 16 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Cartilage)
Permeabilization
Yes - Triton X-100
Specification
Cartilage
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Dr. Tina Yuan

Verified customer

Submitted Jan 15 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (cartilage)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: proteinase K 10ug/ml
Permeabilization
No
Specification
cartilage
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 24°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Aug 16 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Bone Marrow)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization
No
Specification
Bone Marrow
Blocking step
Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative
10% NBF

Premier Laboratory

Verified customer

Submitted May 04 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Knee)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization
No
Specification
Knee
Blocking step
Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative
10%NBF fix, 10% Formic Acid decalcification

Premier Laboratory

Verified customer

Submitted May 04 2016

Answer

Antibody ab58632 is our recommendation, given the number of references that have used it for IHC of FFPE tissue. Here is a link to the list of references, annotated "IHC-P" for paraffin.

https://www.abcam.com/collagen-x-antibody-ab58632-references.html

The four other antibodies that have been tested in this application should be effective, at least for the species that are listed on the datasheets, but they do not have as much data. My second choice would be the mouse monoclonal ab49945, depending on the species of your samples. For instance, I would not recommend it for staining mouse tissue, if there is endogenous mouse IgG in the sample that a secondary anti-mouse IgG antibody might react with. However, two references do have data from immuno-compromised mice.

Read More

Question
Answer

Thank you for contacting us. The anti-Collagen X antibody ab58632 is a whole serum antibody and the concentration has not been determined. Generally concentrations are only relevant for purified antibodies, since products from ascites or whole serum may contains proteins other than the target protein. We recommend that you follow the suggested dilution for your application, which for IHC is 1/50-1/100.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab49945with the order number 1115958. You can expect to receive this on July 2nd, but to check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

1-10 of 18 Abreviews or Q&A

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