Anti-Collagen X antibody (ab58632)
Key features and details
- Rabbit polyclonal to Collagen X
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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Overview
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Product name
Anti-Collagen X antibody
See all Collagen X primary antibodies -
Description
Rabbit polyclonal to Collagen X -
Host species
Rabbit -
Specificity
ab58632 recognizes type X collagen. Exhibits slight cross-reactivity with fibronectin and type II and type IX collagen. Does not cross-react with type I, type III, or type XI collagen. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) Type X collagen from rat chondrosarcoma cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab58632 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/100 - 1/300.
|
Notes |
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WB
1/100 - 1/300. |
Target
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Function
Type X collagen is a product of hyperthrophic chondrotocytes and has been localized to presumptive mineralization zones of hyaline cartilage. -
Involvement in disease
Defects in COL10A1 are the cause of Schmid type metaphyseal chondrodysplasia (SMCD) [MIM:156500]. SMCD is a dominantly inherited disorder of the osseous skeleton. The cardinal features of the phenotype are mild short stature, coxa vara and a waddling gait. Radiography usually shows sclerosis of the ribs, flaring of the metaphyses, and a wide irregular growth plate, especially of the knees. A variant form of SMCD is spondylometaphyseal dysplasia Japanese type. It is characterized by spinal involvement comprising mild platyspondyly, vertebral body abnormalities, and end-plate irregularity. -
Sequence similarities
Contains 1 C1q domain. -
Post-translational
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 1300 Human
- Omim: 120110 Human
- SwissProt: Q03692 Human
- Unigene: 520339 Human
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Alternative names
- COAA1_HUMAN antibody
- Col10a 1 antibody
- COL10A1 antibody
see all
Images
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Anti-Collagen X antibody (ab58632) at 1/500 dilution + HT 1080 (Human fibrosarcoma) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 64,66 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa, 50 kDa, 98 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 150 seconds
The band observed at 64 kDa could potentially be a cleaved form of Collagen X due to the presence of a 18 amino acid signal peptide.
Protocols
Datasheets and documents
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Datasheet download
References (227)
ab58632 has been referenced in 227 publications.
- Wu C et al. Mapk7 deletion in chondrocytes causes vertebral defects by reducing MEF2C/PTEN/AKT signaling. Genes Dis 11:964-977 (2024). WB ; Mouse . PubMed: 37692479
- Li Y et al. Enhancing cartilage repair with optimized supramolecular hydrogel-based scaffold and pulsed electromagnetic field. Bioact Mater 22:312-324 (2023). PubMed: 36263100
- Zhou W et al. High expression COL10A1 promotes breast cancer progression and predicts poor prognosis. Heliyon 8:e11083 (2022). PubMed: 36281404
- Gomez GA et al. Contrasting effects of Ksr2, an obesity gene, on trabecular bone volume and bone marrow adiposity. Elife 11:N/A (2022). PubMed: 36342465
- Ma Z et al. Mechanical Unloading of Engineered Human Meniscus Models Under Simulated Microgravity: A Transcriptomic Study. Sci Data 9:736 (2022). PubMed: 36450785