Immunocytochemistry/ Immunofluorescence abreview for Anti-Collagen X antibody

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Primary Chondrocytes)
Specification
Primary Chondrocytes
Fixative
Paraformaldehyde
Permeabilization
Yes - TritonX-100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: 21°C

Other product details

Dilution
1/50
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 7.5% BSA

Secondary antibody

Name
Non-Abcam antibody was used: Molecular Probes SKU# A-21206
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500

Additional data

Additional Notes
This antibody performed nicely.
Image is of primary chondrocytes grown on glass. Green is Col X, and nuclei are visualized with DAPI in blue.

Mr. Steve Bond

Verified customer

Submitted Oct 31 2008

Sign up