Immunohistochemistry (Frozen sections) abreview for Anti-Collagen X antibody

Good
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (P17 Mouse Metatarsal Bone)
Specification
P17 Mouse Metatarsal Bone
Fixative
Paraformaldehyde
Permeabilization
Yes - PBS+Tween 20
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 4°C

Other product details

Dilution
1/50
Incubation time
8 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking Buffer

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti Rabbit conjugated with Alexa 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000

Additional data

Additional Notes
Antigen retrieval was done by incubating 20min in 95C hot bath
Picture was taken at Hypertrophy Zone.

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Submitted Mar 19 2010

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