Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen X antibody

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (cartilage)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: proteinase K 10ug/ml
Permeabilization
No
Specification
cartilage
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 24°C
Fixative
Formaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: Vector Laboratory ABC Reagent
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
this antibody stained the cartilage matrix in the hypertrophic zone of growth plate, which is composed of collagen X

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Submitted Aug 16 2016

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