Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen X antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Cartilage)
Antigen retrieval step
Enzymatic
Permeabilization
Yes - Proteinase K
Specification
Cartilage
Blocking step
4% BSA+ 4% horse serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: OVERNIGHT
Dilution
1/2000

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: Anti-rabbit IgG biotinylated species-specific whol
Host species: Donkey
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
Antigen retrieval and permeabilization was performed in just one step with proteinase K. Photo: 1. Negative control. 2. Positive control: mouse knee joint.

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Submitted Sep 12 2018

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