Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen X antibody

Inconclusive
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mouse growth plate)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric buffer ph6
Permeabilization
No
Specification
mouse growth plate
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 22°C
Fixative
Formaldehyde

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: immpress kit
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Abcam response
Thank you for participating in our AbTrial program and your valuable feedback. As the product had not yet been tested in this application, we were unsure what the result might be, and we appreciate the time and effort that the reviewer has put in to testing this product in this application. We encourage all researchers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program.

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Verified customer

Submitted Nov 02 2018

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