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    collagen-x-antibody-col-10-ab49945.pdf

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Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Proteins Collagen
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Anti-Collagen X antibody [COL-10] (ab49945)

  • Datasheet
  • SDS
Reviews (4)Q&A (4)References (55)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen X antibody [COL-10] (ab49945)
  • Immunocytochemistry/ Immunofluorescence - Anti-Collagen X antibody [COL-10] (ab49945)

Key features and details

  • Mouse monoclonal [COL-10] to Collagen X
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgM

Overview

  • Product name

    Anti-Collagen X antibody [COL-10]
    See all Collagen X primary antibodies
  • Description

    Mouse monoclonal [COL-10] to Collagen X
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    See all applications and species data
  • Immunogen

    Purified pig Collagen type X.

  • Epitope

    Hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with porcine collagen type X.
  • Positive control

    • IHC-P: Enzyme treated sections of decalcified human osteochondroma.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.097% Sodium azide
    Constituent: 99% Ascites
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    COL-10
  • Isotype

    IgM
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Collagen

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgM H&L (ab9167)
    • Rabbit Anti-Mouse IgM mu chain (ab9175)
  • Isotype control

    • Mouse IgM [B11/7] - Isotype control (ab91545)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab49945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
Application Abreviews Notes
IHC-P (3)
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Notes
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.

Target

  • Function

    Type X collagen is a product of hyperthrophic chondrotocytes and has been localized to presumptive mineralization zones of hyaline cartilage.
  • Involvement in disease

    Defects in COL10A1 are the cause of Schmid type metaphyseal chondrodysplasia (SMCD) [MIM:156500]. SMCD is a dominantly inherited disorder of the osseous skeleton. The cardinal features of the phenotype are mild short stature, coxa vara and a waddling gait. Radiography usually shows sclerosis of the ribs, flaring of the metaphyses, and a wide irregular growth plate, especially of the knees. A variant form of SMCD is spondylometaphyseal dysplasia Japanese type. It is characterized by spinal involvement comprising mild platyspondyly, vertebral body abnormalities, and end-plate irregularity.
  • Sequence similarities

    Contains 1 C1q domain.
  • Post-translational
    modifications

    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession Q03692 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1300 Human
    • Omim: 120110 Human
    • SwissProt: Q03692 Human
    • Unigene: 520339 Human
    • Alternative names

      • COAA1_HUMAN antibody
      • Col10a 1 antibody
      • COL10A1 antibody
      • Collagen alpha 1(X) chain antibody
      • Collagen alpha-1(X) chain antibody
      • Collagen type X alpha 1 (Schmid metaphyseal chondrodysplasia) antibody
      • Collagen type X alpha 1 antibody
      • Collagen X alpha 1 polypeptide antibody
      • CollagenX antibody
      • fa66d11 antibody
      • fb10c08 antibody
      • OTTHUMP00000040411 antibody
      • Procollagen type X alpha 1 antibody
      • Schmid metaphyseal chondrodysplasia antibody
      • wu:fa66d11 antibody
      • wu:fb10c08 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen X antibody [COL-10] (ab49945)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen X antibody [COL-10] (ab49945)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ostochondroma tissue labelling Collagen X with ab49945 at a dilution of 1/1000.

      This image was generated using the ascites version of the product.

    • Immunocytochemistry/ Immunofluorescence - Anti-Collagen X antibody [COL-10] (ab49945)
      Immunocytochemistry/ Immunofluorescence - Anti-Collagen X antibody [COL-10] (ab49945)

      ICC/IF of HT1080 human fibrosarcoma cells labelling Collagen X with ab49945 at 1/1000 dilution. The cells were methanol acetone fixed. Secondary antibody was a Cy3 anti mouse IgG. Nuclei are stained with DAPI.

      This image was generated using the ascites version of the product.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (55)

    Publishing research using ab49945? Please let us know so that we can cite the reference in this datasheet.

    ab49945 has been referenced in 55 publications.

    • Ledo AM  et al. Extracellular matrix mechanics regulate transfection and SOX9-directed differentiation of mesenchymal stem cells. Acta Biomater 110:153-163 (2020). PubMed: 32417266
    • Park DY  et al. Engineered cartilage utilizing fetal cartilage-derived progenitor cells for cartilage repair. Sci Rep 10:5722 (2020). PubMed: 32235934
    • Yan W  et al. Platelet-rich plasma combined with injectable hyaluronic acid hydrogel for porcine cartilage regeneration: a 6-month follow-up. Regen Biomater 7:77-90 (2020). PubMed: 32153994
    • Hu N  et al. Chondrogenic induction of human osteoarthritic cartilage-derived mesenchymal stem cells activates mineralization and hypertrophic and osteogenic gene expression through a mechanomiR. Arthritis Res Ther 21:167 (2019). PubMed: 31287025
    • Zhao X  et al. MicroRNA-495 enhances chondrocyte apoptosis, senescence and promotes the progression of osteoarthritis by targeting AKT1. Am J Transl Res 11:2232-2244 (2019). PubMed: 31105831
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-8 of 8 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen X antibody [COL-10]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Human bone marrow derived stromal cells transplant)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
    Permeabilization
    No
    Specification
    Human bone marrow derived stromal cells transplant
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 15 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen X antibody [COL-10]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Growth plate cartilage)
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: 700U/ml hyaluronidase
    Permeabilization
    Yes - 0.2% Triton X 100 in TBS
    Specification
    Growth plate cartilage
    Blocking step
    10% goat serum + 1% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Mr. Chris Erickson

    Verified customer

    Submitted Jan 06 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen X antibody [COL-10]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rabbit Tissue sections (Cartilage)
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: hyaluronidase
    Permeabilization
    Yes - triton X-100
    Specification
    Cartilage
    Blocking step
    BSA as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 28 2015

    Immunohistochemistry (Frozen sections) abreview for Anti-Collagen X antibody [COL-10]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (femur (embryonic))
    Permeabilization
    Yes - 40%OCT
    Specification
    femur (embryonic)
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 04 2015

    Question

    a customer of ours is interested in your ab49945 but would like to know which is the epitope recognise by this Ab.
    The customer is looking for an antibody able to recognise the central part of the protein (not N-term and not C-term).

    Thanks in advance and kind regards

    Read More

    Abcam community

    Verified customer

    Asked on Nov 22 2012

    Answer

    Thank you for contacting us.

    I am sorry we do not have an anybody to the central region of collagen X protein.

    Unfortunately the epitope of ab4995 has not been determined so we are unable to confirm which part of protein this ab binds.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Abcam Scientific Support

    Answered on Nov 22 2012

    Question

    I would like a test code for trying ab49945 on FFPE decalcified Rabbit Femur. Rabbit is not currently listed as a reactive species.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 08 2011

    Answer

    DISCOUNT CODE: xxx Expiration date: xxx I am very pleased to hear you would like to accept our offer and test ab49945 on rabbit. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for rabbit and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Dec 08 2011

    Question

    I would like to test ab49945 for reactivity to rabbit.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 08 2011

    Answer

    I am very pleased to hear you would like to accept our offer and test ab49945 in rabbit. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for rabbit and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Sep 08 2011

    Question

    On the protocol for the type X collagen antibody (ab49945) it states the antigen retreival step is enzyme treatment. Please can you clarify the details of the enzyme treatment in terms of enzyme type, concentration, temperature and time? Thanks.

    Read More

    Abcam community

    Verified customer

    Asked on Jan 17 2008

    Answer

    Thank you for your enquiry. According to our files, the samples were treated first with 0.2% hyaluronidase for 1 hour at 37 deg C and then treated with 0.1% protease for 9 minutes at 37 deg C. I hope this information will be useful. Should you require any other assistance, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Jan 17 2008

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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