Anti-Collagen X antibody [COL-10] (ab49945)
Key features and details
- Mouse monoclonal [COL-10] to Collagen X
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgM
Overview
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Product name
Anti-Collagen X antibody [COL-10]
See all Collagen X primary antibodies -
Description
Mouse monoclonal [COL-10] to Collagen X -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Purified pig Collagen type X.
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Epitope
Hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with porcine collagen type X. -
Positive control
- IHC-P: Enzyme treated sections of decalcified human osteochondroma.
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General notes
This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.01% Sodium azide
Constituent: 0.142% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
COL-10 -
Isotype
IgM -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab49945 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (3) |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Type X collagen is a product of hyperthrophic chondrotocytes and has been localized to presumptive mineralization zones of hyaline cartilage. -
Involvement in disease
Defects in COL10A1 are the cause of Schmid type metaphyseal chondrodysplasia (SMCD) [MIM:156500]. SMCD is a dominantly inherited disorder of the osseous skeleton. The cardinal features of the phenotype are mild short stature, coxa vara and a waddling gait. Radiography usually shows sclerosis of the ribs, flaring of the metaphyses, and a wide irregular growth plate, especially of the knees. A variant form of SMCD is spondylometaphyseal dysplasia Japanese type. It is characterized by spinal involvement comprising mild platyspondyly, vertebral body abnormalities, and end-plate irregularity. -
Sequence similarities
Contains 1 C1q domain. -
Post-translational
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 1300 Human
- Omim: 120110 Human
- SwissProt: Q03692 Human
- Unigene: 520339 Human
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Alternative names
- COAA1_HUMAN antibody
- Col10a 1 antibody
- COL10A1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ostochondroma tissue labelling Collagen X with ab49945 at a dilution of 1/1000.
This image was generated using the ascites version of the product.
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ICC/IF of HT1080 human fibrosarcoma cells labelling Collagen X with ab49945 at 1/1000 dilution. The cells were methanol acetone fixed. Secondary antibody was a Cy3 anti mouse IgG. Nuclei are stained with DAPI.
This image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (87)
ab49945 has been referenced in 87 publications.
- Wu C et al. Mapk7 deletion in chondrocytes causes vertebral defects by reducing MEF2C/PTEN/AKT signaling. Genes Dis 11:964-977 (2024). IHC-Fr ; Mouse . PubMed: 37692479
- Cheng C et al. Repair of a Rat Mandibular Bone Defect by Hypertrophic Cartilage Grafts Engineered From Human Fractionated Adipose Tissue. Front Bioeng Biotechnol 10:841690 (2022). PubMed: 35350180
- Browe DC et al. Promoting endogenous articular cartilage regeneration using extracellular matrix scaffolds. Mater Today Bio 16:100343 (2022). PubMed: 35865410
- Peniche Silva CJ et al. Enthesis: not the same in each localisation - a molecular, histological and biomechanical study. Eur Cell Mater 44:43-55 (2022). PubMed: 35976149
- Huang RL et al. Engineering hypertrophic cartilage grafts from lipoaspirate for critical-sized calvarial bone defect reconstruction: An adipose tissue-based developmental engineering approach. Bioeng Transl Med 7:e10312 (2022). PubMed: 36176620