Overview

  • Product name

    Anti-Collagen X antibody [EPR13044]
    See all Collagen X primary antibodies
  • Description

    Rabbit monoclonal [EPR13044] to Collagen X
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Collagen X aa 550-650. The exact sequence is proprietary.
    Database link: Q03692

  • Positive control

    • WB: Human fetal skin, placenta and cartilage tissue lysates; Rat skin and cartilage tissue lysates; Mouse cartilage tissue lysate
  • General notes

    A trial size is available to purchase for this antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13044
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab182563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).

Target

  • Function

    Type X collagen is a product of hyperthrophic chondrotocytes and has been localized to presumptive mineralization zones of hyaline cartilage.
  • Involvement in disease

    Defects in COL10A1 are the cause of Schmid type metaphyseal chondrodysplasia (SMCD) [MIM:156500]. SMCD is a dominantly inherited disorder of the osseous skeleton. The cardinal features of the phenotype are mild short stature, coxa vara and a waddling gait. Radiography usually shows sclerosis of the ribs, flaring of the metaphyses, and a wide irregular growth plate, especially of the knees. A variant form of SMCD is spondylometaphyseal dysplasia Japanese type. It is characterized by spinal involvement comprising mild platyspondyly, vertebral body abnormalities, and end-plate irregularity.
  • Sequence similarities

    Contains 1 C1q domain.
  • Post-translational
    modifications

    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • COAA1_HUMAN antibody
    • Col10a 1 antibody
    • COL10A1 antibody
    • Collagen alpha 1(X) chain antibody
    • Collagen alpha-1(X) chain antibody
    • Collagen type X alpha 1 (Schmid metaphyseal chondrodysplasia) antibody
    • Collagen type X alpha 1 antibody
    • Collagen X alpha 1 polypeptide antibody
    • CollagenX antibody
    • fa66d11 antibody
    • fb10c08 antibody
    • OTTHUMP00000040411 antibody
    • Procollagen type X alpha 1 antibody
    • Schmid metaphyseal chondrodysplasia antibody
    • wu:fa66d11 antibody
    • wu:fb10c08 antibody
    see all

Images

  • All lanes : Anti-Collagen X antibody [EPR13044] (ab182563) at 1/1000 dilution

    Lane 1 : Human fetal skin tissue lysates
    Lane 2 : Rat skin tissue lysates
    Lane 3 : Mouse cartilage tissue lysates
    Lane 4 : Human cartilage tissue lysates
    Lane 5 : Rat cartilage tissue lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 66 kDa
    Observed band size: 66 kDa



    Exposure time
    Lane 1: 6 seconds
    Lane 2: 70 seconds
    Lane 3 to 5: 10 seconds

    Blocking/Diluting buffer and concentration 5% NFDM/TBST

    Collagen X is normally not expressed in human healthy cartilage but its expression is detected at protein and mRNA level in human osteoarthritic cartilage according to PMID 22178514 and 26861791.

  • All lanes : Anti-Collagen X antibody [EPR13044] (ab182563) at 1/1000 dilution

    Lane 1 : Human fetal skin lysate
    Lane 2 : Human placenta lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 66 kDa
    Additional bands at: 66 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:

  • Wang P  et al. Histone deacetylase-4 and histone deacetylase-8 regulate interleukin-1ß-induced cartilage catabolic degradation through MAPK/JNK and ERK pathways. Int J Mol Med 41:2117-2127 (2018). Read more (PubMed: 29393346) »
  • Chen L  et al. MiR-29b-3p promotes chondrocyte apoptosis and facilitates the occurrence and development of osteoarthritis by targeting PGRN. J Cell Mol Med 21:3347-3359 (2017). WB . Read more (PubMed: 28609022) »
See all 7 Publications for this product

Customer reviews and Q&As

Question
Answer

The information about positive control can be found in publications e.g. spleen cells and lymphocytes has been successfully used in http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2630710/

NIH3T3 cells in http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1132157/

Mouse chondrocytes in; http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC2120322&blobtype=pdf

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up