Recombinant Anti-Collagen X antibody [EPR13044] (ab182563)
![Western blot - Anti-Collagen X antibody [EPR13044] (ab182563)](https://www.abcam.com/ps/products/182/ab182563/Images/ab182563-324118-anti-collagen-x-antibody-epr13044-western-blot.jpg "Western blot - Anti-Collagen X antibody [EPR13044] (ab182563)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13044] to Collagen X
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Collagen X antibody [EPR13044]
See all Collagen X primary antibodies -
Description
Rabbit monoclonal [EPR13044] to Collagen X -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Collagen X aa 550-650. The exact sequence is proprietary.
Database link: Q03692 -
Positive control
- WB: Human fetal skin, placenta and cartilage tissue lysates; Rat skin and cartilage tissue lysates; Mouse cartilage tissue lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13044 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab182563 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/2000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa). |
Target
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Function
Type X collagen is a product of hyperthrophic chondrotocytes and has been localized to presumptive mineralization zones of hyaline cartilage. -
Involvement in disease
Defects in COL10A1 are the cause of Schmid type metaphyseal chondrodysplasia (SMCD) [MIM:156500]. SMCD is a dominantly inherited disorder of the osseous skeleton. The cardinal features of the phenotype are mild short stature, coxa vara and a waddling gait. Radiography usually shows sclerosis of the ribs, flaring of the metaphyses, and a wide irregular growth plate, especially of the knees. A variant form of SMCD is spondylometaphyseal dysplasia Japanese type. It is characterized by spinal involvement comprising mild platyspondyly, vertebral body abnormalities, and end-plate irregularity. -
Sequence similarities
Contains 1 C1q domain. -
Post-translational
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 1300 Human
- Entrez Gene: 12813 Mouse
- Entrez Gene: 25681 Rat
- Omim: 120110 Human
- SwissProt: Q03692 Human
- SwissProt: Q05306 Mouse
- Unigene: 520339 Human
- Unigene: 443177 Mouse
see all -
Alternative names
- COAA1_HUMAN antibody
- Col10a 1 antibody
- COL10A1 antibody
see all
Images
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Western blot - Anti-Collagen X antibody [EPR13044] (ab182563)All lanes : Anti-Collagen X antibody [EPR13044] (ab182563) at 1/1000 dilution
Lane 1 : Human fetal skin tissue lysates
Lane 2 : Rat skin tissue lysates
Lane 3 : Mouse cartilage tissue lysates
Lane 4 : Human cartilage tissue lysates
Lane 5 : Rat cartilage tissue lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 66 kDa
Observed band size: 66 kDaExposure time
Lane 1: 6 seconds
Lane 2: 70 seconds
Lane 3 to 5: 10 secondsBlocking/Diluting buffer and concentration 5% NFDM/TBST
Collagen X is normally not expressed in human healthy cartilage but its expression is detected at protein and mRNA level in human osteoarthritic cartilage according to PMID 22178514 and 26861791.
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![Western blot - Anti-Collagen X antibody [EPR13044] (ab182563)](https://www.abcam.com/ps/products/182/ab182563/Images/ab182563-213941-anti-collagen-x-antibody-epr13044-western-blot.jpg)
Western blot - Anti-Collagen X antibody [EPR13044] (ab182563)All lanes : Anti-Collagen X antibody [EPR13044] (ab182563) at 1/1000 dilution
Lane 1 : Human fetal skin lysate
Lane 2 : Human placenta lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 66 kDa
Additional bands at: 66 kDa. We are unsure as to the identity of these extra bands. -
![Anti-Collagen X antibody [EPR13044] (ab182563)](https://www.abcam.com/ps/products/182/ab182563/Images/ab182563-3-benefits-of-recombinant-antibodies.png)
Anti-Collagen X antibody [EPR13044] (ab182563)
Datasheets and documents
Certificate of Compliance
References (10)
ab182563 has been referenced in 10 publications.
- Wu Y et al. High methylation of lysine acetyltransferase 6B is associated with the Cobb angle in patients with congenital scoliosis. J Transl Med 18:210 (2020). PubMed: 32448279
- Rubí-Sans G et al. Development of a Three-Dimensional Bioengineered Platform for Articular Cartilage Regeneration. Biomolecules 10:N/A (2019). PubMed: 31905668
- Zhang X et al. The in vivo chondrogenesis of cartilage stem/progenitor cells from auricular cartilage and the perichondrium. Am J Transl Res 11:2855-2865 (2019). PubMed: 31217859
- Wang P et al. Histone deacetylase-4 and histone deacetylase-8 regulate interleukin-1ß-induced cartilage catabolic degradation through MAPK/JNK and ERK pathways. Int J Mol Med 41:2117-2127 (2018). PubMed: 29393346
- Chen L et al. MiR-29b-3p promotes chondrocyte apoptosis and facilitates the occurrence and development of osteoarthritis by targeting PGRN. J Cell Mol Med 21:3347-3359 (2017). WB . PubMed: 28609022
- Recha-Sancho L et al. Dedifferentiated Human Articular Chondrocytes Redifferentiate to a Cartilage-Like Tissue Phenotype in a Poly(e-Caprolactone)/Self-Assembling Peptide Composite Scaffold. Materials (Basel) 9:N/A (2016). PubMed: 28773609
- Recha-Sancho L & Semino CE Heparin based self-assembling peptide scaffold reestablish chondrogenic phenotype of expanded de-differentiated human chondrocytes. J Biomed Mater Res A N/A:N/A (2016). Human . PubMed: 26939919
- Recha-Sancho L & Semino CE Chondroitin Sulfate- and Decorin-Based Self-Assembling Scaffolds for Cartilage Tissue Engineering. PLoS One 11:e0157603 (2016). WB ; Human . PubMed: 27315119
- Fernández-Muiños T et al. Bimolecular based heparin and self-assembling hydrogel for tissue engineering applications. Acta Biomater 16:35-48 (2015). WB ; Human . PubMed: 25595471
- Amano K et al. Indian hedgehog signaling regulates transcription and expression of collagen type X via Runx2/Smads interactions. J Biol Chem 289:24898-910 (2014). WB ; Mouse . PubMed: 25028519
