Recombinant
RabMAb

Recombinant Anti-Collagen X antibody [EPR13044] - BSA and Azide free (ab232661)

Overview

  • Product name

    Anti-Collagen X antibody [EPR13044] - BSA and Azide free
    See all Collagen X primary antibodies
  • Description

    Rabbit monoclonal [EPR13044] to Collagen X - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Collagen X aa 550-650. The exact sequence is proprietary.
    Database link: Q03692

  • Positive control

    • WB: Human fetal skin and placenta lysates.
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®). 

    ab232661 is a PBS-only buffer format of ab182563. Please refer to ab182563 for recommended dilutions, protocols, and image data.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13044
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab232661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 66 kDa.

Target

  • Function

    Type X collagen is a product of hyperthrophic chondrotocytes and has been localized to presumptive mineralization zones of hyaline cartilage.
  • Involvement in disease

    Defects in COL10A1 are the cause of Schmid type metaphyseal chondrodysplasia (SMCD) [MIM:156500]. SMCD is a dominantly inherited disorder of the osseous skeleton. The cardinal features of the phenotype are mild short stature, coxa vara and a waddling gait. Radiography usually shows sclerosis of the ribs, flaring of the metaphyses, and a wide irregular growth plate, especially of the knees. A variant form of SMCD is spondylometaphyseal dysplasia Japanese type. It is characterized by spinal involvement comprising mild platyspondyly, vertebral body abnormalities, and end-plate irregularity.
  • Sequence similarities

    Contains 1 C1q domain.
  • Post-translational
    modifications

    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • COAA1_HUMAN antibody
    • Col10a 1 antibody
    • COL10A1 antibody
    • Collagen alpha 1(X) chain antibody
    • Collagen alpha-1(X) chain antibody
    • Collagen type X alpha 1 (Schmid metaphyseal chondrodysplasia) antibody
    • Collagen type X alpha 1 antibody
    • Collagen X alpha 1 polypeptide antibody
    • CollagenX antibody
    • fa66d11 antibody
    • fb10c08 antibody
    • OTTHUMP00000040411 antibody
    • Procollagen type X alpha 1 antibody
    • Schmid metaphyseal chondrodysplasia antibody
    • wu:fa66d11 antibody
    • wu:fb10c08 antibody
    see all

Images

  • All lanes : Anti-Collagen X antibody [EPR13044] (ab182563) at 1/1000 dilution

    Lane 1 : Human fetal skin lysate
    Lane 2 : Human placenta lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 66 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182563).

References

ab232661 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab232661.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up