Recombinant
RabMAb

Recombinant Anti-Collagen XVII antibody [EPR14758] - BSA and Azide free (ab239005)

Overview

  • Product name

    Anti-Collagen XVII antibody [EPR14758] - BSA and Azide free
    See all Collagen XVII primary antibodies
  • Description

    Rabbit monoclonal [EPR14758] to Collagen XVII - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Collagen XVII aa 1-100. The exact sequence is proprietary.
    Database link: Q9UMD9

  • Positive control

    • IHC-P: Human skin tissue.
  • General notes

    Ab239005 is the carrier-free version of ab186415. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab239005 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14758
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab239005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance

    Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in the gene coding for collagen XVII are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD 1, is generated by proteolytic processing of the full length form. Two transcript variants, one resulting from alternative splicing in the 3' UTR, have been identified for this gene.
  • Cellular localization

    Cell junction, hemidesmosome. Membrane; Single-pass type II membrane protein. Note=Localized along the plasma membrane of the hemidesmosome. 120 kDa linear IgA disease antigen and 97 kDa linear IgA disease antigen: Secreted, extracellular space, extracellular matrix, basement membrane.
  • Database links

  • Alternative names

    • 180 kDa bullous pemphigoid antigen 2 antibody
    • Alpha 1 type XVII collagen antibody
    • BA16H23.2 antibody
    • BP 180 antibody
    • BP180 antibody
    • BPA 2 antibody
    • BPAG 2 antibody
    • BPAG2 antibody
    • Bullous pemphigoid antigen 2 antibody
    • COL17A1 antibody
    • Collagen 17 antibody
    • Collagen alpha 1 XVII chain antibody
    • Collagen alpha 1(XVII) chain antibody
    • Collagen alpha1 XVII chain antibody
    • Collagen type XVII alpha 1 antibody
    • Collagen XVII alpha 1 polypeptide antibody
    • CollagenXVII antibody
    • Epidermolysis bullosa junctional localisata variant antibody
    • FLJ60881 antibody
    • KIAA0204 antibody
    • LAD 1 antibody
    • LAD1 antibody
    see all

Images

  • Immunofluorescent analysis of 4% paraformaldehyde fixed A431 cells labeling Collagen XVII using ab186415 at a 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution. DAPI counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186415).

  • Immunohistochemical analysis of paraffin embedded Human skin tissue sections labeling Collagen XVII using ab186415 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186415).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

References

ab239005 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab239005.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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