Immunohistochemistry (Frozen sections) abreview for Anti-Collagen XVII antibody [EPR18614]

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Human skin)
Permeabilization
Yes - 0.5% Triton TBS Ca/Azide
Specification
Human skin
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: 5% BSA TBS Ca/Azide
Dilution
1/100

Secondary antibody

Dilution
1/800
Name
Non-Abcam antibody was used: Life Technologies Donkey anti-rabbit IgG Alexa 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Abcam response
We would like to thank the reviewer for submitting this AbReview for this product. As the product had not yet been tested in immunohistochemistry with frozen sections (IHC-Fr), we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application. We encourage all researchers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program.

Mr. Tyler Crowe

Verified customer

Submitted Sep 24 2018

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