Overview

  • Product name

    Collagenase Activity Assay Kit (Colorimetric)
    See all Collagenase kits
  • Detection method

    Colorimetric
  • Sample type

    Cell Lysate, Purified protein
  • Assay type

    Enzyme activity
  • Sensitivity

    > 20 µU
  • Assay time

    0h 25m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Bacteria
  • Product overview

    Collagenase Activity Assay Kit (ab196999) provides a quick and easy way to determine activity of Collagenase. The assay measures collagenase activity using a synthetic peptide (FALGPA) that mimics the structure of collagen. It is suitable for measuring activity of bacterial collagenases such as from Clostridium histolyticum type I-XI. This assay can also be used for screening or characterization of collagenase inhibitors.


    The limit of detection for this assay is 0.02 mU Collagenase.


    Collagenase assay protocol summary:
    - add samples and controls to wells
    - add reaction mix
    - analyze with microplate reader in kinetic mode for 5-15 min

  • Notes

    Collagenase (EC 3.4.24.3) is an enzyme in the matrix metalloproteinase family that breaks down collagen, assisting in degradation of the extracellular matrix, which is a key step in the pathogenesis of bacteria. Collagen is an abundant structural protein present in the connective tissue of animals. Collagenase has been used clinically for the treatment of Dupuytren’s contracture, an affliction characterized by a thickening of connective tissue.

  • Platform

    Microplate reader

Properties

Images

  • Collagenase activity over 10 minutes.

  • Enzyme activity of collagenase and inhibition by 10 mM (1,10)-Phenanthroline. Assay was performed following the kit protocol.

Protocols

References

This product has been referenced in:

  • Roda G  et al. Ripe and Raw Pu-Erh Tea: LC-MS Profiling, Antioxidant Capacity and Enzyme Inhibition Activities of Aqueous and Hydro-Alcoholic Extracts. Molecules 24:N/A (2019). Read more (PubMed: 30699941) »
See 1 Publication for this product

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