Overview

  • Product name

  • Description

    Rabbit polyclonal to Collagenase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Dot blot, ICC/IFmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Full length native protein (purified) corresponding to Collagenase. (Isolated and purified from Clostridium histolyticum).

Properties

Applications

Our Abpromise guarantee covers the use of ab182916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/100000. Predicted molecular weight: 126 kDa.
Dot blot 1/10000 - 1/100000.
ICC/IF 1/10000 - 1/100000.

Target

  • Relevance

    Function: Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. Domain: There are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases). The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. PTM: Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase. Similarity: Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains.
  • Cellular localization

    Secreted, extracellular space, extracellular matrix
  • Alternative names

    • CLG antibody
    • Fibroblast collagenase antibody
    • matrix metallopeptidase 1 (interstitial collagenase) antibody
    • matrix metallopeptidase 1 antibody
    • MMP-1 antibody
    see all

References

ab182916 has not yet been referenced specifically in any publications.

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