Anti-COLQ antibody (ab127048)
Key features and details
- Rabbit polyclonal to COLQ
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-COLQ antibody -
Description
Rabbit polyclonal to COLQ -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 248-420 of Human COLQ (UniProt Q9Y215).
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Positive control
- HepG2 whole cell lysate, Human colon carcinoma tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab127048 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 48 kDa.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 48 kDa. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Anchors the catalytic subunits of asymmetric AChE to the synaptic basal lamina. -
Tissue specificity
Found at the end plate of skeletal muscle. -
Involvement in disease
Defects in COLQ are the cause of congenital myasthenic syndrome Engel type (CMSE) [MIM:603034]; also known as end-plate acetylcholinesterase deficiency or congenital myasthenic syndrome type IC (CMS-IC). CMSE is a rare autosomal recessive congenital myasthenic syndrome characterized by onset during childhood, generalized weakness, abnormal fatigability on exertion, refrectoriness to acetylcholinesterase drugs, decremental electromyographic response and morphological abnormalities of the neuromuscular junctions. -
Sequence similarities
Belongs to the COLQ family.
Contains 2 collagen-like domains. -
Domain
The proline-rich attachment domain (PRAD) binds the AChE catalytic subunits. -
Post-translational
modificationsThe triple-helical tail is stabilized by disulfide bonds at each end. -
Cellular localization
Cell junction > synapse. - Information by UniProt
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Database links
- Entrez Gene: 8292 Human
- Omim: 603033 Human
- SwissProt: Q9Y215 Human
- Unigene: 146735 Human
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Alternative names
- Acetylcholinesterase collagen-like tail subunit isoform I antibody
- Acetylcholinesterase collagenic tail peptide antibody
- Acetylcholinesterase collagenic tail peptide precursor antibody
see all
Images
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Anti-COLQ antibody (ab127048) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 48 kDa
10% SDS PAGE -
ab1267048, at a dilution of 1/500, staining COLQ in paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab127048 has not yet been referenced specifically in any publications.