Overview

  • Product name

    Anti-COLQ antibody
  • Description

    Rabbit polyclonal to COLQ
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 248-420 of Human COLQ (UniProt Q9Y215).

  • Positive control

    • HepG2 whole cell lysate, Human colon carcinoma tissue
  • General notes

    Keep as concentrated solution.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab127048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 48 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Anchors the catalytic subunits of asymmetric AChE to the synaptic basal lamina.
  • Tissue specificity

    Found at the end plate of skeletal muscle.
  • Involvement in disease

    Defects in COLQ are the cause of congenital myasthenic syndrome Engel type (CMSE) [MIM:603034]; also known as end-plate acetylcholinesterase deficiency or congenital myasthenic syndrome type IC (CMS-IC). CMSE is a rare autosomal recessive congenital myasthenic syndrome characterized by onset during childhood, generalized weakness, abnormal fatigability on exertion, refrectoriness to acetylcholinesterase drugs, decremental electromyographic response and morphological abnormalities of the neuromuscular junctions.
  • Sequence similarities

    Belongs to the COLQ family.
    Contains 2 collagen-like domains.
  • Domain

    The proline-rich attachment domain (PRAD) binds the AChE catalytic subunits.
  • Post-translational
    modifications

    The triple-helical tail is stabilized by disulfide bonds at each end.
  • Cellular localization

    Cell junction > synapse.
  • Information by UniProt
  • Database links

  • Alternative names

    • Acetylcholinesterase collagen-like tail subunit isoform I antibody
    • Acetylcholinesterase collagenic tail peptide antibody
    • Acetylcholinesterase collagenic tail peptide precursor antibody
    • Acetylcholinesterase-associated collagen antibody
    • AChE Q subunit antibody
    • asymmetric acetylcholinesterase antibody
    • Collagen-like tail subunit (single strand of homotrimer) of asymmetric acetylcholinesterase antibody
    • Colq antibody
    • COLQ_HUMAN antibody
    • EAD antibody
    • OTTHUMP00000209566 antibody
    • OTTHUMP00000209567 antibody
    • single strand of homotrimeric collagen-like tail subunit of asymmetric acetylcholinesterase antibody
    see all

Images

  • Anti-COLQ antibody (ab127048) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 48 kDa



    10% SDS PAGE
  • ab1267048, at a dilution of 1/500, staining COLQ in paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry.

References

ab127048 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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