Key features and details
- Rabbit polyclonal to COLQ
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-COLQ antibody
DescriptionRabbit polyclonal to COLQ
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment, corresponding to a region within amino acids 248-420 of Human COLQ (UniProt Q9Y215).
- HepG2 whole cell lysate, Human colon carcinoma tissue
General notesKeep as concentrated solution.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab127048 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 48 kDa.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionAnchors the catalytic subunits of asymmetric AChE to the synaptic basal lamina.
Tissue specificityFound at the end plate of skeletal muscle.
Involvement in diseaseDefects in COLQ are the cause of congenital myasthenic syndrome Engel type (CMSE) [MIM:603034]; also known as end-plate acetylcholinesterase deficiency or congenital myasthenic syndrome type IC (CMS-IC). CMSE is a rare autosomal recessive congenital myasthenic syndrome characterized by onset during childhood, generalized weakness, abnormal fatigability on exertion, refrectoriness to acetylcholinesterase drugs, decremental electromyographic response and morphological abnormalities of the neuromuscular junctions.
Sequence similaritiesBelongs to the COLQ family.
Contains 2 collagen-like domains.
DomainThe proline-rich attachment domain (PRAD) binds the AChE catalytic subunits.
modificationsThe triple-helical tail is stabilized by disulfide bonds at each end.
Cellular localizationCell junction > synapse.
- Information by UniProt
- Acetylcholinesterase collagen-like tail subunit isoform I antibody
- Acetylcholinesterase collagenic tail peptide antibody
- Acetylcholinesterase collagenic tail peptide precursor antibody
Anti-COLQ antibody (ab127048) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 48 kDa
10% SDS PAGE
ab1267048, at a dilution of 1/500, staining COLQ in paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry.
ab127048 has not yet been referenced specifically in any publications.