Key features and details
- Rabbit polyclonal to COMMD9
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-COMMD9 antibody
DescriptionRabbit polyclonal to COMMD9
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
antigen sequence, corresponding to amino acids 41-171 of Human COMMD9.
- COMMD9-transfected HEK293T lysate; Human small intestine tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121303 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
Sequence similaritiesContains 1 COMM domain.
- Information by UniProt
- COMD9_HUMAN antibody
- COMM domain containing 9 antibody
- COMM domain-containing protein 9 antibody
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus and golgi apparatus. Recommended concentration of ab121303 1-4 µg/ml. Cells treated with PFA/Triton X-100.
All lanes : Anti-COMMD9 antibody (ab121303) at 1/250 dilution
Lane 1 : vector only transfected HEK293T lysate
Lane 2 : COMMD9 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells
Developed using the ECL technique.
10-20% SDS PAGE
ab121303 at 1/100 dilution staining COMMD9 in Paraffin-embedded Human small intestine tissue by Immunohistochemistry.
ab121303 has not yet been referenced specifically in any publications.