Key features and details
- Rabbit polyclonal to COMP/Cartilage oligomeric matrix protein
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-COMP/Cartilage oligomeric matrix protein antibody
See all COMP/Cartilage oligomeric matrix protein primary antibodies
DescriptionRabbit polyclonal to COMP/Cartilage oligomeric matrix protein
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow
Recombinant fragment (His-T7-tag) corresponding to Human COMP/Cartilage oligomeric matrix protein aa 123-240. (Expressed in E.coli).
SHCTDVNECNAHPCFPRVRCINTSPGFRCEACPPGYSGPTHQGVGLAFAK ANKQVCTDINECETGQHNCVPNSVCINTRGSFQCGPCQPGFVGDQASGCQ RRAQRFCPDGSPSECHEH
Database link: P49747
- WB: Recombinant human COMP/Cartilage oligomeric matrix protein; Human and mouse cartilage tissue lysate. IHC-P: Human testis tissue.
This product was previously labelled as COMP
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231977 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 83 kDa.|
FunctionMay play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7.
Tissue specificityAbundantly expressed in the chondrocyte extracellular matrix, and is also found in bone, tendon, ligament and synovium and blood vessels. Increased amounts are produced during late stages of osteoarthritis in the area adjacent to the main defect.
Involvement in diseaseDefects in COMP are the cause of multiple epiphyseal dysplasia type 1 (EDM1) [MIM:132400]. EDM is a generalized skeletal dysplasia associated with significant morbidity. Joint pain, joint deformity, waddling gait, and short stature are the main clinical signs and symptoms. EDM is broadly categorized into the more severe Fairbank and the milder Ribbing types.
Defects in COMP are the cause of pseudoachondroplasia (PSACH) [MIM:177170]. PSAC is a dominantly inherited chondrodysplasia characterized by short stature and early-onset osteoarthrosis. PSACH is more severe than EDM1 and is recognized in early childhood.
Sequence similaritiesBelongs to the thrombospondin family.
Contains 4 EGF-like domains.
Contains 1 TSP C-terminal (TSPC) domain.
Contains 8 TSP type-3 repeats.
Developmental stagePresent during the earliest stages of limb maturation and is later found in regions where the joints develop.
DomainThe cell attachment motif mediates the attachment to chondrocytes. It mediates the induction of both the IAP family of survival proteins and the antiapoptotic response.
The TSP C-terminal domain mediates interaction with FN1 and ACAN.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia 1, multiple) antibody
- Cartilage oligomeric matrix protein antibody
- Cartilage oligomeric matrix protein precursor antibody
Formalin-fixed, paraffin-embedded human testis tissue stained for COMP/Cartilage oligomeric matrix protein using ab231977 at 20 µg/mLin immunohistochemical analysis. DAB staining.
Anti-COMP/Cartilage oligomeric matrix protein antibody (ab231977) at 3 µg/ml + Recombinant human COMP/Cartilage oligomeric matrix protein
HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 83 kDa
All lanes : Anti-COMP/Cartilage oligomeric matrix protein antibody (ab231977) at 3 µg/ml
Lane 1 : Human cartilage tissue lysate
Lane 2 : Mouse cartilage tissue lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 83 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231977 has been referenced in 3 publications.
- Tessier S et al. Arp2/3 inactivation causes intervertebral disc and cartilage degeneration with dysregulated TonEBP-mediated osmoadaptation. JCI Insight 5:N/A (2020). PubMed: 31961823
- Gorth DJ et al. A New Understanding of the Role of IL-1 in Age-Related Intervertebral Disc Degeneration in a Murine Model. J Bone Miner Res N/A:N/A (2019). PubMed: 30875127
- Rosas S et al. Cartilage oligomeric matrix protein in patients with osteoarthritis is independently associated with metastatic disease in prostate cancer. Oncotarget 10:4776-4785 (2019). PubMed: 31413818