Overview

  • Product name

    Anti-COMP/Cartilage oligomeric matrix protein antibody
    See all COMP/Cartilage oligomeric matrix protein primary antibodies
  • Description

    Rabbit polyclonal to COMP/Cartilage oligomeric matrix protein
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Frmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to Human COMP/Cartilage oligomeric matrix protein aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab74523)

  • Positive control

    • Recombinant Human COMP/Cartilage oligomeric matrix protein (ab124873) can be used as a positive control in WB. This antibody gave a positive signal in human skeletal muscle and human heart tissue lysates.
  • General notes

     This product was previously labelled as COMP

     

Properties

Applications

Our Abpromise guarantee covers the use of ab74524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 82 kDa).
IHC-Fr Use at an assay dependent concentration. PubMed: 21939397

Target

  • Function

    May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7.
  • Tissue specificity

    Abundantly expressed in the chondrocyte extracellular matrix, and is also found in bone, tendon, ligament and synovium and blood vessels. Increased amounts are produced during late stages of osteoarthritis in the area adjacent to the main defect.
  • Involvement in disease

    Defects in COMP are the cause of multiple epiphyseal dysplasia type 1 (EDM1) [MIM:132400]. EDM is a generalized skeletal dysplasia associated with significant morbidity. Joint pain, joint deformity, waddling gait, and short stature are the main clinical signs and symptoms. EDM is broadly categorized into the more severe Fairbank and the milder Ribbing types.
    Defects in COMP are the cause of pseudoachondroplasia (PSACH) [MIM:177170]. PSAC is a dominantly inherited chondrodysplasia characterized by short stature and early-onset osteoarthrosis. PSACH is more severe than EDM1 and is recognized in early childhood.
  • Sequence similarities

    Belongs to the thrombospondin family.
    Contains 4 EGF-like domains.
    Contains 1 TSP C-terminal (TSPC) domain.
    Contains 8 TSP type-3 repeats.
  • Developmental stage

    Present during the earliest stages of limb maturation and is later found in regions where the joints develop.
  • Domain

    The cell attachment motif mediates the attachment to chondrocytes. It mediates the induction of both the IAP family of survival proteins and the antiapoptotic response.
    The TSP C-terminal domain mediates interaction with FN1 and ACAN.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia 1, multiple) antibody
    • Cartilage oligomeric matrix protein antibody
    • Cartilage oligomeric matrix protein precursor antibody
    • COMP antibody
    • COMP_HUMAN antibody
    • EDM 1 antibody
    • EDM1 antibody
    • EPD 1 antibody
    • EPD1 antibody
    • Epiphyseal dysplasia 1 antibody
    • Epiphyseal dysplasia 1 multiple antibody
    • Epiphyseal dysplasia multiple 1 antibody
    • MED antibody
    • MGC13181 antibody
    • MGC149768 antibody
    • PSACH antibody
    • pseudoachondroplasia (epiphyseal dysplasia 1, multiple) antibody
    • Pseudoachondroplasia antibody
    • THBS 5 antibody
    • THBS5 antibody
    • Thrombospondin 5 antibody
    • Thrombospondin-5 antibody
    • Thrombospondin5 antibody
    • TSP5 antibody
    see all

Images

  • All lanes : Anti-COMP/Cartilage oligomeric matrix protein antibody (ab74524) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate
    Lane 2 : Human heart tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 82 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab74524 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406

References

This product has been referenced in:

See all 7 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human cartilage and bone)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Specification
Human cartilage and bone
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 488µg/mL · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 25 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Horse Tissue sections (cartilage)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K in 0.05 M Tris-HCl, pH 7,6
Permeabilization
No
Specification
cartilage
Fixative
Formaldehyde

Marjan Steppe

Verified customer

Submitted Dec 07 2016

Answer

Thank you for contacting us.

The antibody will most likely be okay. If you have some samples that aren't too precious, you may want to try a WB or even dot blot to see if you get reactivity. Storage at 4C is not ideal, but the antibody may not have lost reactivity during this time.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Answer

Thank you for contacting Abcam.

The anti-COMP antibody, ab74524 has not been tested in immunofluoresce. If we have tested an antibody in a particular application and it failed to give any signal, then we will put that information on the datasheet for the antibody.

If there is anything else I can help you with, please let me know.

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Answer

Thank you for your reply.

As ab42256 is specific peptide, there is no alternative for it. Also, with this product there is currently a 1-2 week wait until we are able to send it out. With this product, we only begin the restocking process once an order has been placed. Therefore, if your customer would like it as soon as possible, I would recommend that you place the order as soon as possible.

If there is anything else I can help you with, please let me know.

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Answer

Thank you for contacting Abcam.

Please find a list of possible alternatives below:

ab7973 alternative
https://www.abcam.com/Bcl2-antibody-ab18210.html
https://www.abcam.com/Bcl2-antibody-ab47489.html
https://www.abcam.com/Bcl2-antibody-100-D5-ab692.html

ab3611 alternative
https://www.abcam.com/RAGE-antibody-ab30381.html
https://www.abcam.com/RAGE-antibody-ab30381.html

ab66103 alternatives
https://www.abcam.com/Proteinase-Activated-Receptor-4-antibody-ab56359.html

ab74524 alternatives
https://www.abcam.com/COMP-antibody-MA37C94-HC484D1-ab11056.html
https://www.abcam.com/COMP-antibody-EPR62892-ab128893.html

ab125428 alternatives
https://www.abcam.com/Dynein-light-chain-antibody-ab37464.html
https://www.abcam.com/Dynein-light-chain-antibody-ab116064.html

ab42256 - No Alternative.

If there is anything else I can help you with, please let me know.

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