Recombinant
RabMAb

Anti-Complement C9 antibody [EPR11232-82] (ab173302)

Overview

  • Product name
    Anti-Complement C9 antibody [EPR11232-82]
  • Description
    Rabbit monoclonal [EPR11232-82] to Complement C9
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human Complement C9. The exact sequence is proprietary.
    Database link: P02748

  • Positive control
    • Human angioneoplasm, Human serum and Human plasma lysates, Human colon and Human spleen lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11232-82
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab173302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 72 kDa (predicted molecular weight: 63 kDa).
IHC-P 1/100 - 1/200.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function
      Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC.
    • Tissue specificity
      Plasma.
    • Involvement in disease
      Defects in C9 are a cause of complement component 9 deficiency (C9D) [MIM:613825]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
    • Sequence similarities
      Belongs to the complement C6/C7/C8/C9 family.
      Contains 1 EGF-like domain.
      Contains 1 LDL-receptor class A domain.
      Contains 1 MACPF domain.
      Contains 1 TSP type-1 domain.
    • Post-translational
      modifications
      Thrombin cleaves factor C9 to produce C9a and C9b.
      Phosphorylation sites are present in the extracelllular medium.
    • Cellular localization
      Secreted. Cell membrane. Secreted as soluble monomer. Oligomerizes at target membranes, forming a pre-pore. A conformation change then leads to the formation of a 100 Angstrom diameter pore.
    • Information by UniProt
    • Database links
    • Alternative names
      • C9 antibody
      • CO9_HUMAN antibody
      • Complement component C9b antibody

    Images

    • All lanes : Anti-Complement C9 antibody [EPR11232-82] (ab173302) at 1/1000 dilution

      Lane 1 : Human angioneoplasm lysate
      Lane 2 : Human serum lysate
      Lane 3 : Human plasma lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 63 kDa
      Additional bands at: 72 kDa. We are unsure as to the identity of these extra bands.

    • Immunoprecipitation. ab173302 at 1/1000 labeling Complement C9 immunoprecipitated from Human serum using ab173302 at 1/10.

    • Immunohistochemical analysis of paraffin embedded Human colon tissue labeling Complement C9 with ab173302 at 1/100.

    • Immunohistochemical analysis of paraffin embedded Human spleen tissue labeling Complement C9 with ab173302 at 1/100.

    References

    This product has been referenced in:
    • Tougan T  et al. Molecular Camouflage of Plasmodium falciparum Merozoites by Binding of Host Vitronectin to P47 Fragment of SERA5. Sci Rep 8:5052 (2018). Read more (PubMed: 29567995) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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