Overview

  • Product name

    Anti-Complement C9 antibody [EPR11232-82]
    See all Complement C9 primary antibodies
  • Description

    Rabbit monoclonal [EPR11232-82] to Complement C9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human Complement C9. The exact sequence is proprietary.
    Database link: P02748

  • Positive control

    • WB: Human serum lysates; IHC-P: human colon and spleen tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11232-82
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab173302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 72 kDa (predicted molecular weight: 63 kDa).
IHC-P 1/10000.

See IHC antigen retrieval protocols.

For unpurified use at 1/100 - 1/200.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC.
    • Tissue specificity

      Plasma.
    • Involvement in disease

      Defects in C9 are a cause of complement component 9 deficiency (C9D) [MIM:613825]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
    • Sequence similarities

      Belongs to the complement C6/C7/C8/C9 family.
      Contains 1 EGF-like domain.
      Contains 1 LDL-receptor class A domain.
      Contains 1 MACPF domain.
      Contains 1 TSP type-1 domain.
    • Post-translational
      modifications

      Thrombin cleaves factor C9 to produce C9a and C9b.
      Phosphorylation sites are present in the extracelllular medium.
    • Cellular localization

      Secreted. Cell membrane. Secreted as soluble monomer. Oligomerizes at target membranes, forming a pre-pore. A conformation change then leads to the formation of a 100 Angstrom diameter pore.
    • Information by UniProt
    • Database links

    • Alternative names

      • C9 antibody
      • CO9_HUMAN antibody
      • Complement component C9b antibody

    Images

    • Anti-Complement C9 antibody [EPR11232-82] (ab173302) at 1/10000 dilution (Purified) + Human serum lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 63 kDa
      Observed band size: 60-70 kDa
      why is the actual band size different from the predicted?



      The molecular weight observed is consistent with what has been described in PMID: 29767720

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling Complement C9 with purified ab173302 at 1/10,000 dilution (0.07 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunohistochemical analysis of paraffin embedded Human colon tissue labeling Complement C9 with ab173302 (unpurified) at 1/100.

    • Immunohistochemical analysis of paraffin embedded Human spleen tissue labeling Complement C9 with ab173302 (unpurified) at 1/100.

    References

    This product has been referenced in:

    • Deleon-Pennell KY  et al. Glycoproteomic Profiling Provides Candidate Myocardial Infarction Predictors of Later Progression to Heart Failure. ACS Omega 4:1272-1280 (2019). Read more (PubMed: 30729226) »
    • Zhao P  et al. The imbalance in the complement system and its possible physiological mechanisms in patients with lung cancer. BMC Cancer 19:201 (2019). Read more (PubMed: 30841875) »
    See all 3 Publications for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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