Recombinant
RabMAb

Recombinant Anti-Complement factor B antibody [EPR9288(B)] (ab133765)

Overview

  • Product name

    Anti-Complement factor B antibody [EPR9288(B)]
    See all Complement factor B primary antibodies
  • Description

    Rabbit monoclonal [EPR9288(B)] to Complement factor B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within the C terminal end of Human Complement factor B (UniProt ID: P00751).

  • Positive control

    • Human serum and plasma
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Dissociation constant (KD)

    KD = 1.43 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR9288(B)
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab133765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 95, 60 kDa (predicted molecular weight: 86 kDa).
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Relevance

      Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes.
    • Cellular localization

      Plasma membrane and Secreted
    • Database links

    • Alternative names

      • C3/C5 convertase antibody
      • Complement factor B antibody
      • Glycine-rich beta glycoprotein antibody
      • PBF2 antibody
      • Properdin factor B antibody
      see all

    Images

    • All lanes : Anti-Complement factor B antibody [EPR9288(B)] (ab133765) at 1/1000 dilution

      Lane 1 : Human serum
      Lane 2 : Human plasma

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 86 kDa
      Observed band size: 95 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 60 kDa (possible isoform)

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab133765 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133765.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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