Recombinant Anti-Complement factor B antibody [EPR9288(B)] (ab133765)
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Overview
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Product name
Anti-Complement factor B antibody [EPR9288(B)]
See all Complement factor B primary antibodies -
Description
Rabbit monoclonal [EPR9288(B)] to Complement factor B -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to a region within the C terminal end of Human Complement factor B (UniProt ID: P00751).
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Positive control
- Human serum and plasma
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Dissociation constant (KD)
KD = 1.43 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9288(B) -
Isotype
IgG
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab133765 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 95, 60 kDa (predicted molecular weight: 86 kDa). |
Target
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Relevance
Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes. -
Cellular localization
Plasma membrane and Secreted -
Database links
- Entrez Gene: 629 Human
- Omim: 138470 Human
- SwissProt: P00751 Human
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Alternative names
- C3/C5 convertase antibody
- Complement factor B antibody
- Glycine-rich beta glycoprotein antibody
see all
Images
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![Western blot - Anti-Complement factor B antibody [EPR9288(B)] (ab133765)](https://www.abcam.com/ps/products/133/ab133765/Images/ab133765-147882-anti-complement-factor-b-antibody-epr9288b-western-blot.jpg)
Western blot - Anti-Complement factor B antibody [EPR9288(B)] (ab133765)All lanes : Anti-Complement factor B antibody [EPR9288(B)] (ab133765) at 1/1000 dilution
Lane 1 : Human serum
Lane 2 : Human plasma
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 86 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa (possible isoform) -
![Other - Anti-Complement factor B antibody [EPR9288(B)] (ab133765)](https://www.abcam.com/ps/products/133/ab133765/Images/ab133765-147881-anti-complement-factor-b-antibody-epr9288b.jpg)
Other - Anti-Complement factor B antibody [EPR9288(B)] (ab133765)
Protocols
Datasheets and documents
References
ab133765 has not yet been referenced specifically in any publications.
