RecombinantRabMAb

Recombinant Anti-Complement factor B antibody [EPR9288(B)] (ab133765)

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Western blot - Anti-Complement factor B antibody [EPR9288(B)] (ab133765)
  • OI-RD Scanning - Anti-Complement factor B antibody [EPR9288(B)] (ab133765)
  • Anti-Complement factor B antibody [EPR9288(B)] (ab133765)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9288(B)] to Complement factor B
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Complement factor B antibody [EPR9288(B)]
    See all Complement factor B primary antibodies

  • Description

    Rabbit monoclonal [EPR9288(B)] to Complement factor B

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to a region within the C terminal end of Human Complement factor B (UniProt ID: P00751).

  • Positive control

    • Human serum and plasma

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Dissociation constant (KD)

    KD = 1.43 x 10 -10 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR9288(B)

  • Isotype

    IgG

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 95, 60 kDa (predicted molecular weight: 86 kDa).
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 95, 60 kDa (predicted molecular weight: 86 kDa).
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Relevance

      Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes.

    • Cellular localization

      Plasma membrane and Secreted

    • Database links

      • Alternative names

        • C3/C5 convertase antibody
        • Complement factor B antibody
        • Glycine-rich beta glycoprotein antibody
        • PBF2 antibody
        • Properdin factor B antibody
        see all

      Images

      • Western blot - Anti-Complement factor B antibody [EPR9288(B)] (ab133765)
        Western blot - Anti-Complement factor B antibody [EPR9288(B)] (ab133765)
        All lanes : Anti-Complement factor B antibody [EPR9288(B)] (ab133765) at 1/1000 dilution

        Lane 1 : Human serum
        Lane 2 : Human plasma

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

        Predicted band size: 86 kDa
        Observed band size: 95 kDa
        why is the actual band size different from the predicted?
        Additional bands at: 60 kDa (possible isoform)

      • OI-RD Scanning - Anti-Complement factor B antibody [EPR9288(B)] (ab133765)
        OI-RD Scanning - Anti-Complement factor B antibody [EPR9288(B)] (ab133765)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD
      • Anti-Complement factor B antibody [EPR9288(B)] (ab133765)
        Anti-Complement factor B antibody [EPR9288(B)] (ab133765)

      References (1)

      Publishing research using ab133765? Please let us know so that we can cite the reference in this datasheet.

      ab133765 has been referenced in 1 publication.

      • Hayashi N  et al. Multiple biomarkers of sepsis identified by novel time-lapse proteomics of patient serum. PLoS One 14:e0222403 (2019). PubMed: 31568522

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