Recombinant Anti-Complement factor B antibody [EPR9288(B)] (ab133765)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9288(B)] to Complement factor B
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-Complement factor B antibody [EPR9288(B)]
See all Complement factor B primary antibodies -
Description
Rabbit monoclonal [EPR9288(B)] to Complement factor B -
Host species
Rabbit -
Tested Applications & Species
Application Species WB Human -
Immunogen
Synthetic peptide corresponding to a region within the C terminal end of Human Complement factor B (UniProt ID: P00751).
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Positive control
- Human serum and plasma
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Dissociation constant (KD)
KD = 1.43 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9288(B) -
Isotype
IgG
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133765 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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WB |
Human
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Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 95, 60 kDa (predicted molecular weight: 86 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 95, 60 kDa (predicted molecular weight: 86 kDa). |
Target
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Relevance
Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes. -
Cellular localization
Plasma membrane and Secreted -
Database links
- Entrez Gene: 629 Human
- Omim: 138470 Human
- SwissProt: P00751 Human
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Alternative names
- C3/C5 convertase antibody
- Complement factor B antibody
- Glycine-rich beta glycoprotein antibody
see all
Images
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All lanes : Anti-Complement factor B antibody [EPR9288(B)] (ab133765) at 1/1000 dilution
Lane 1 : Human serum
Lane 2 : Human plasma
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 86 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa (possible isoform)
Datasheets and documents
References (1)
ab133765 has been referenced in 1 publication.
- Hayashi N et al. Multiple biomarkers of sepsis identified by novel time-lapse proteomics of patient serum. PLoS One 14:e0222403 (2019). PubMed: 31568522