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The sample extraction is native so care needs to be taken to extract the samples at equivalent starting concentrations to maintain the protein : detergent ratio and maintain enzyme integrity and activity.
Step 3 – in this we recommend adjusting the lysed cell sample protein concentration to approximately 5.5 mg/mL as determined by some form of protein assay (you can take a small % of sample for this – it may need some prior lysis with SDS or other detergent based on the nature of the sample or protein assay conducted).
Step 4 -Once adjusted it is time for the protein extraction using our supplied extraction detergent.
Wewould recommend doing both steps – step 3 adjustment is precautionary – step 4 is essential to normalize different samples loaded into the plate wells,
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Mar 26 2012