Question (41373) | Complex I Enzyme Activity Microplate Assay Kit (Colorimetric) (ab109721)

Go to datasheet (ab109721)


How the sample should be prepared?


Thank you for contacting us.

The sample extraction is native so care needs to be taken to extract the samples at equivalent starting concentrations to maintain the protein : detergent ratio and maintain enzyme integrity and activity.

Step 3 – in this we recommend adjusting the lysed cell sample protein concentration to approximately 5.5 mg/mL as determined by some form of protein assay (you can take a small % of sample for this – it may need some prior lysis with SDS or other detergent based on the nature of the sample or protein assay conducted).

Step 4 -Once adjusted it is time for the protein extraction using our supplied extraction detergent.

Wewould recommend doing both steps – step 3 adjustment is precautionary – step 4 is essential to normalize different samples loaded into the plate wells,

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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