Question (70116) | Complex I Enzyme Activity Microplate Assay Kit (Colorimetric) (ab109721)

Go to datasheet (ab109721)

Question

I have one more question: I need to so this assay under reducing conditions, eg with addition of DTT to the mitochondrial preparation. Would that interfer with the assay for either complex I or II?

Answer


We haven't specifically tested the complex I or II kits under reducing conditions and cannot guarantee that it will not affect the enzyme activities. With that being said, my colleagues believe that a reducing agent at moderate concentration shouldn't affect the assay capture step significantly, especially if the reduced samples are sufficiently diluted in the wells.

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