Question (70563) | Complex I Enzyme Activity Microplate Assay Kit (Colorimetric) (ab109721)

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Question

are there steps i can take is my protein concentration is low? i am using cultured cardiomyocytes and already have the samples prepared

Answer

Thank you for contacting us.

If the stating protein concentration is low, the detergent can be scaled down to use less, so instead of 10X dilution, 20X or 40X dilution could be used but I wouldn’t go below 40X.

If the sample is already extracted in detergent there isn’t much to do but perhaps add more to the well, 250-200uL and incubate longer.

Please let me know if you have any further questions!

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