Question (72103) | Complex I Enzyme Activity Microplate Assay Kit (Colorimetric) (ab109721)

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Question

Inquiry: Complex I Enzyme Activity Microplate Assay Kit (ab109721) May use trypsin to detach the cells or it can interfire with the elisa assay?

Answer

Thank you for your enquiry and interest in our products.

I can confirm that enzymatic digestion of the cultured cells with trypsin+EDTA does not interfere with this particular assay (ab109721: Complex I Enzyme Activity Microplate Assay Kit) as long as the trypsin is quenched soon after the cells detached from the plate and a good viability count is obtained.

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