Question (72494) | Complex I Enzyme Activity Microplate Assay Kit (Colorimetric) (ab109721)

Go to datasheet (ab109721)


starting sample is purified mitochondria, is centrifugation after incubation with the detergent, in Step 6.5 (20' at 12000xg) necessary. Won't this pellet mitochondria?


In step 6.4, the mitochondria will be lysed. Step 6.5 is required to pellet insolubles post-lysis. Save the pellet but it should not contain anything of value assuming lysis was successful.

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