Question (72494) | Complex I Enzyme Activity Microplate Assay Kit (Colorimetric) (ab109721)

Go to datasheet (ab109721)

Question

starting sample is purified mitochondria, is centrifugation after incubation with the detergent, in Step 6.5 (20' at 12000xg) necessary. Won't this pellet mitochondria?

Answer

In step 6.4, the mitochondria will be lysed. Step 6.5 is required to pellet insolubles post-lysis. Save the pellet but it should not contain anything of value assuming lysis was successful.

Sign up