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Is it necessary to isolate mitochondria or can I use total protein?
Asked on Mar 14 2013
You can use total tissue homogenate or cultured cell lysate. These will have lower activity per mass of protein of course.
Figures 2 and 3 show activity derived from whole cell lysates in cultured HepG2 (human liver derived), human fibroblasts and rat cardiomyocytes.
Answered on Mar 14 2013