Question (72905) | Complex I Enzyme Activity Microplate Assay Kit (Colorimetric) (ab109721)

Go to datasheet (ab109721)

Question

Is it necessary to isolate mitochondria or can I use total protein?

Answer

You can use total tissue homogenate or cultured cell lysate. These will have lower activity per mass of protein of course.

Figures 2 and 3 show activity derived from whole cell lysates in cultured HepG2 (human liver derived), human fibroblasts and rat cardiomyocytes.

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