Question (74477) | Complex I Enzyme Activity Microplate Assay Kit (Colorimetric) (ab109721)

Go to datasheet (ab109721)

Question

Is the RIPA buffer compatible with this kit?
What is the recommended lysis buffer?

Answer

I would not recommend RIPA buffer because it contains SDS a powerful ionic detergent. The MS141 ab109721 Complex I activity assay contains a mild non-ionic detergent lauryl (n-dodecyl) maltoside which preserves Complex I activity.

Please check the sample preparation guide as well:

http://www.mitosciences.com/PDF/mitosciences-sample-preparation-guide.pdf

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