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Is the RIPA buffer compatible with this kit?
What is the recommended lysis buffer?
Asked on Jul 19 2013
I would not recommend RIPA buffer because it contains SDS a powerful ionic detergent. The MS141 ab109721 Complex I activity assay contains a mild non-ionic detergent lauryl (n-dodecyl) maltoside which preserves Complex I activity.
Please check the sample preparation guide as well:
Padamjeet SinghAbcam Scientific Support
Answered on Jul 19 2013