HCT116 cells were cultivated in standard DMEM medium for 48 hrs. Cell preparation was performed as described in the protocol. Protein concentration in each sample was quantified and adjusted by Bradford assay. We looked at complex I activity dependent on the protein amount applied to the plate (fig.) thus we would suggest to use protein amounts around 200 µg for this assay. The kit is easy to use but takes a long time to perform. The results seem to be reliable.
Mr. Christian Marx
Submitted Dec 12 2013
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