Overview

  • Product name
    Complex I Immunocapture Kit
    See all Complex I kits
  • Assay type
    Quantitative
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Product overview

    250 µg, 500 µg or 750 µg monoclonal antibody irreversibly crosslinked to protein G-agarose beads which can immunocapture ~60 µg, ~120 µg or ~180 µg respectively of Complex I from heart mitochondria.


    ab109711 allows for isolation of Complex I from small amounts of tissue. This facilitates subsequent analysis of assembly state and activity. Thus the enzyme retains NADH-ferricyanide, NADH-CoQ1 and NADH hexaaminoruthenium reductase activities after isolation, and with added lipids it also shows significant rotenone sensitivity. Finally, the extent of post translational modifications including oxidative damage can be readily analyzed by proteomic approaches or antibody detection of these modifications. Uses for ab109711 include but are not limited to examining alterations of Complex I subunits in inherited mitochondrial diseases, Parkinson's disease, Alzheimer's disease, ALS, schizophrenia, and aging.


    Note: The immunocapture protocol for this kit requires Abcam detergent (lauryl maltoside, ab109857).


    Product supplied as a 6.25% slurry. For the 250 µg, this consists of 250 µg antibody conjugated to 25 µL beads in a volume of 400 µL.

  • Tested applications
    Suitable for: IPmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab109711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.

Images

  • Immunoprecipitation - Complex I Immunocapture Kit (ab109711)
    Immunoprecipitation - Complex I Immunocapture Kit (ab109711)
    Complex I immunoprecipitation using antibody ab109798/MS101c crosslinked to protein G-agarose beads as product ab109711

Protocols

References

This product has been referenced in:
  • Bridges HR  et al. Subunit NDUFV3 is present in two distinct isoforms in mammalian complex I. Biochim Biophys Acta 1858:197-207 (2017). Read more (PubMed: 27940020) »
  • Jian B  et al. Resveratrol restores sirtuin 1 (SIRT1) activity and pyruvate dehydrogenase kinase 1 (PDK1) expression after hemorrhagic injury in a rat model. Mol Med 20:10-6 (2014). Functional Studies ; Rat . Read more (PubMed: 24395567) »

See all 16 Publications for this product

Publishing research using ab109711? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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12 reviews or Q&A

10
Votes: Highest First

need protocol and advice on how much to order. I see the information that states how much Complex I is pulled down, but this doesn't mean all at once does it?

Hello! Below is some more information about kit sizes. Please let me know if I can be of further assistance.

The kits size is based on antibody amount not the beads amount. 250ug refers to 25ul beads, 500ug to 50ul beads and 750ug to 75ul bead...

Read More

I would appreciate if you can give me also
The catalogue number of plain beads that is
Mentionned end of page 13 of ab109711-complex
I immunocapture kit guide


The lab let me know that both ab109711 and ab109798 are IP antibodies - unfortunately they do not work in WB. One can use it to pull down the whole complex I but needs to use other complex I subunit antibodies to blot the corresponding targets. Read More

10% Lauryl Maltoside diluent.

To prepare 10% lauryl maltoside as the detergent for ab109711, pure water (deionized, nanopure etc) can be used. The lauryl maltoside should go into solution fairly easily. Gently turn in rotator, try not to shake it too much to prevent frothing.
...

Read More

What is the recommended ratio of sample:beads to use?



In general, for well-prepped mitochondria, the ratio is 1-2 mg of mitochondria per 10 uL of beads. The optimal conditions will depend on the quality and source of the mitochondria so it’s very difficult to be specific, however. A good q...

Read More

Hi,

I would like to know more details ASAP about:
1-elution buffer composition that you recommend in the kit such as 1% SDS.
Is it a buffer or just a water based solution where SDS is solubilized..
2-Second to run a gel you deffini...

Read More

Hello! Please find the answers to your questions below.

1-elution buffer composition that you recommend in the kit such as 1% SDS.
Is it a buffer or just a water based solution where SDS is solubilized..



It is a water ba...

Read More

Customer kindly called because they were getting no bands in IP using the kit.

Thank you for contacting Abcam regarding A109711.

I have been in contact with the lab regarding your experiences with this product. However as you have tried a number of the troubleshooting steps already they have requested that I ask you fo...

Read More

adipose tissue
protocol prep suggestions and guidance on protocol changes for using adipose tissue with this product

Thank you for contacting us.



I have contacted our mitochondria-experts in the lab and received the following reply:

We don’t have a lot of experience with adipose tissue. My suggestion would be therefore to isolate...

Read More

1) Of the 3 elution methods (Glycine, SDS, Urea), which is the best method recommended by Abcam?

2) If Glycine buffer elution method is used, how do I neutralized the acidic pH?

Thank you for your enquiry.

The three different elution methods are recommended to different purposes. Proteins eluted with Glycine remain activity, which is suitable for follow up activity assays. Plus the antibody beads can still be resued...

Read More

Can I confirm the following things about this Complex I Immunocapture Kit?

1) This product only contains antibodies crosslinked to agarose beads, and nothing else. The name "Immunocapture Kit" was confusing as I thought there were other comp...

Read More

Thank you for your enquiry. Please find the answers to your questions below.

1) Thank you for your feedback. We will take this into consideration with the production team.

2) Typically customers have used this kit in combination w...

Read More

I would like to ask what is the minimum amount of mouse brain tissue required for Immunocapture kit Complex I (as well as complex III, and IV) so as to pull down Complex I. We would need enough material for running an SDS gel.

The MitoSciences laboratory recommends the following: Use 1 mg of brain tissue lysate, eluting into the minimum volume possible. A glycine buffer elution procedure allows the reuse of the beads. If you wish to analyze the product by Weste...

Read More

1-10 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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