Complex II Immunocapture Kit (ab109799)

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Overview

  • Product name

    Complex II Immunocapture Kit
    See all Complex II kits

  • Assay type

    Quantitative

  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human

  • Product overview

    250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~13 µg, ~26 µg or ~39 µg respectively of Complex II from heart mitochondria. Isolated mitochondria are available from several species for use as control samples.


     


    The Complex II Immunocapture Kit allows isolation of the succinate dehydrogenase complex from small amounts of tissue. This facilitates subsequent analysis of assembly state, activity and the extent of post translational modifications including oxidative damage that occur with aging. Uses for the Complex II immunocapture kit include research on aging genetic mitochondrial disease, and various cancers due to mutations in this enzyme complex such as paragangliomas and phaeo-chromocytomas.


     


    Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).


     


     

  • Notes

    Related products

    Review the mitochondrial assay guide, or the full metabolism assay guide to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.

Properties

Images

  • Immunoprecipitation - Complex II Immunocapture Kit (ab109799)
    Immunoprecipitation - Complex II Immunocapture Kit (ab109799)

    Complex II immunoprecipitation using antibody ab109865  crosslinked to protein G-agarose beads as product ab109799.  Complex II was immunoprecipitated from a lauryl maltoside detergent extract of 1000 ug bovine heart mitochondria or 500 ug human heart mitochondria.  The gel was stained with coomassie brilliant blue.

Protocols

References

This product has been referenced in:

  • Garcia-Diaz B  et al. Infantile encephaloneuromyopathy and defective mitochondrial translation are due to a homozygous RMND1 mutation. Am J Hum Genet 91:729-36 (2012). Read more (PubMed: 23022099) »
  • Finley LW  et al. Succinate dehydrogenase is a direct target of sirtuin 3 deacetylase activity. PLoS One 6:e23295 (2011). IP ; Mouse . Read more (PubMed: 21858060) »
See all 5 Publications for this product

Publishing research using ab109799? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

The protocol says to use at least 5 uL per sample- how many uL are provided per 250 ug vial? How many samples can be run with the 250 ug kit?

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Answer

Thanks for your call today and for your interest in our complex II immunocapture kit.

I've heard back from the lab, and the 250 ug kit will come as as 25 uL solution.

I hope this information is useful, but if there is anything else that we can do for you, please let me know and I'll be happy to help.

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