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    complex-ii-immunocapture-kit-ab109799.pdf

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Signal Transduction Metabolism Mitochondrial
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Complex II Immunocapture Kit (ab109799)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (1)References (7)

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Immunoprecipitation - Complex II Immunocapture Kit (ab109799)

    Key features and details

    • Assay type: Quantitative

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    Overview

    • Product name

      Complex II Immunocapture Kit
      See all Complex II kits
    • Assay type

      Quantitative
    • Species reactivity

      Reacts with: Mouse, Rat, Cow, Human
    • Product overview

      250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~13 µg, ~26 µg or ~39 µg respectively of Complex II from heart mitochondria. Isolated mitochondria are available from several species for use as control samples.


       


      The Complex II Immunocapture Kit allows isolation of the succinate dehydrogenase complex from small amounts of tissue. This facilitates subsequent analysis of assembly state, activity and the extent of post translational modifications including oxidative damage that occur with aging. Uses for the Complex II immunocapture kit include research on aging genetic mitochondrial disease, and various cancers due to mutations in this enzyme complex such as paragangliomas and phaeo-chromocytomas.


       


      Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).


       


       

    • Notes

      Related products

      Review the mitochondrial assay guide, or the full metabolism assay guide to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components 250 µg
      monoclonal Complex II antibody irreversibly cross-linked to protein G-agarose beads 1 x 250µg
    • Research areas

      • Signal Transduction
      • Metabolism
      • Mitochondrial
      • Kits/ Lysates/ Other
      • Kits
      • Cell Metabolism Kits
      • Other Metabolism Assay
      • Metabolism
      • Pathways and Processes
      • Mitochondrial Metabolism
      • Mitochondrial markers
      • Kits/ Lysates/ Other
      • Kits
      • Immunocapture
      • OXPHOS
    • Relevance

      Complex II is also called succinate ubiquinone oxidoreductase or more commonly succinate dehydrogenase complex. This complex is composed of four nuclear encoded subunits and contains a flavin (FAD), non-heme iron centers and a b-type cytochrome as prosthetic groups. It is both a component of the electron transport chain and an enzyme of the Krebs cycle. Complex II deficiencies are seen in OXPHOS genetic disease and found in a type of cancer called paraganglioma.
    • Alternative names

      • SDH
      • Succinate coenzyme Q reductase
      • Succinate dehydrogenase
    • Database links

      • Entrez Gene: 6390 Human
      • Entrez Gene: 6389 Human
      • Entrez Gene: 6391 Human
      • SwissProt: P31040 Human
      • SwissProt: P21912 Human
      • SwissProt: Q99643 Human
      • SwissProt: O14521 Human
      • SwissProt: 6392 Human
      • Unigene: 444472 Human
      • Unigene: 440475 Human
      • Unigene: 465924 Human
      • Unigene: 356270 Human
      see all

    Associated products

    • Related Products

      • 10% Lauryl Maltoside Solution (ab109857)
      • Anti-Complex II Immunocapture antibody [4H12BG12AG2] (ab109865)

    Images

    • Immunoprecipitation - Complex II Immunocapture Kit (ab109799)
      Immunoprecipitation - Complex II Immunocapture Kit (ab109799)

      Complex II immunoprecipitation using antibody ab109865  crosslinked to protein G-agarose beads as product ab109799.  Complex II was immunoprecipitated from a lauryl maltoside detergent extract of 1000 ug bovine heart mitochondria or 500 ug human heart mitochondria.  The gel was stained with coomassie brilliant blue.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (7)

    Publishing research using ab109799? Please let us know so that we can cite the reference in this datasheet.

    ab109799 has been referenced in 7 publications.

    • Jaña F  et al. Complex I and II are required for normal mitochondrial Ca2+ homeostasis. Mitochondrion 49:73-82 (2019). PubMed: 31310854
    • Fernández-Tussy P  et al. miR-873-5p targets mitochondrial GNMT-Complex II interface contributing to non-alcoholic fatty liver disease. Mol Metab 29:40-54 (2019). PubMed: 31668391
    • Garcia-Diaz B  et al. Infantile encephaloneuromyopathy and defective mitochondrial translation are due to a homozygous RMND1 mutation. Am J Hum Genet 91:729-36 (2012). PubMed: 23022099
    • Finley LW  et al. Succinate dehydrogenase is a direct target of sirtuin 3 deacetylase activity. PLoS One 6:e23295 (2011). IP ; Mouse . PubMed: 21858060
    • Paddenberg R  et al. Impact of modulators of mitochondrial ATP-sensitive potassium channel (mitoK(ATP)) on hypoxic pulmonary vasoconstriction. Adv Exp Med Biol 648:361-8 (2009). PubMed: 19536500
    • Ahn BH  et al. A role for the mitochondrial deacetylase Sirt3 in regulating energy homeostasis. Proc Natl Acad Sci U S A 105:14447-52 (2008). PubMed: 18794531
    • Schilling B  et al. Proteomic analysis of succinate dehydrogenase and ubiquinol-cytochrome c reductase (Complex II and III) isolated by immunoprecipitation from bovine and mouse heart mitochondria. Biochim Biophys Acta 1762:213-22 (2006). PubMed: 16120479

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    The protocol says to use at least 5 uL per sample- how many uL are provided per 250 ug vial? How many samples can be run with the 250 ug kit?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 09 2012

    Answer

    Thanks for your call today and for your interest in our complex II immunocapture kit.

    I've heard back from the lab, and the 250 ug kit will come as as 25 uL solution.

    I hope this information is useful, but if there is anything else that we can do for you, please let me know and I'll be happy to help.

    Read More

    Abcam Scientific Support

    Answered on Feb 09 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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