Complex II Immunocapture Kit (ab109799)
- Datasheet
- Specific References (5)
- Protocols
Overview
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Product nameComplex II Immunocapture Kit
See all Complex II kits -
Assay typeQuantitative
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Species reactivityReacts with: Mouse, Rat, Cow, Human
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Product overview
250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~13 µg, ~26 µg or ~39 µg respectively of Complex II from heart mitochondria. Isolated mitochondria are available from several species for use as control samples.
The Complex II Immunocapture Kit allows isolation of the succinate dehydrogenase complex from small amounts of tissue. This facilitates subsequent analysis of assembly state, activity and the extent of post translational modifications including oxidative damage that occur with aging. Uses for the Complex II immunocapture kit include research on aging genetic mitochondrial disease, and various cancers due to mutations in this enzyme complex such as paragangliomas and phaeo-chromocytomas.
Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).
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Tested applicationsSuitable for: IPmore details
Properties
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Components 250 µg monoclonal Complex II antibody irreversibly cross-linked to protein G-agarose beads 1 x 250µg -
Research areas
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RelevanceComplex II is also called succinate ubiquinone oxidoreductase or more commonly succinate dehydrogenase complex. This complex is composed of four nuclear encoded subunits and contains a flavin (FAD), non-heme iron centers and a b-type cytochrome as prosthetic groups. It is both a component of the electron transport chain and an enzyme of the Krebs cycle. Complex II deficiencies are seen in OXPHOS genetic disease and found in a type of cancer called paraganglioma.
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Alternative names
- SDH
- Succinate coenzyme Q reductase
- Succinate dehydrogenase
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Database links
- Entrez Gene: 6390 Human
- Entrez Gene: 6389 Human
- Entrez Gene: 6391 Human
- SwissProt: P31040 Human
- SwissProt: P21912 Human
- SwissProt: Q99643 Human
- SwissProt: O14521 Human
- SwissProt: 6392 Human
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Associated products
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Related Products
Applications
Our Abpromise guarantee covers the use of ab109799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent dilution. |
Images
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Immunoprecipitation - Complex II Immunocapture Kit (ab109799)Complex II immunoprecipitation using antibody ab109865 crosslinked to protein G-agarose beads as product ab109799. Complex II was immunoprecipitated from a lauryl maltoside detergent extract of 1000 ug bovine heart mitochondria or 500 ug human heart mitochondria. The gel was stained with coomassie brilliant blue.
Protocols
References
This product has been referenced in:
- Garcia-Diaz B et al. Infantile encephaloneuromyopathy and defective mitochondrial translation are due to a homozygous RMND1 mutation. Am J Hum Genet 91:729-36 (2012). Read more (PubMed: 23022099) »
- Finley LW et al. Succinate dehydrogenase is a direct target of sirtuin 3 deacetylase activity. PLoS One 6:e23295 (2011). IP ; Mouse . Read more (PubMed: 21858060) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"