Product nameComplex II WB Antibody Cocktail
See all Complex II kits
Species reactivityReacts with: Mouse, Rat, Cow, Human
Contains a total of 305 µg of antibodies and is a pre-mixed solution of 3 monoclonal antibodies against Complex II subunits 70 kDa Fp, 30 kDa Ip along with an antibody against Complex V alpha as a control. This cocktail can be used to verify isolated Complex II purity or to conveniently determine the levels of 2 of the 4 Complex II subunits in cell lines relative to the control protein (Complex V alpha).
Mouse C-V-alpha [15H4C4] monoclonal:
Amount: 50 µg
Working concentration: 1 µg/ml
Mouse C-II-30 (FeS) [21A11AE7] monoclonal:
Amount: 250 µg
Working concentration: 5 µg/ml
Mouse C-II-70 (FL) [2E3GC12FB2AE2] monoclonal:
Amount: 5 µg
Working concentration: 0.1 µg/ml
Tested applicationsSuitable for: WBmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 305 µg Pre-mixed solution of 3 monoclonal antibodies 1 x 305µg
RelevanceComplex II is also called succinate ubiquinone oxidoreductase or more commonly succinate dehydrogenase complex. This complex is composed of four nuclear encoded subunits and contains a flavin (FAD), non-heme iron centers and a b-type cytochrome as prosthetic groups. It is both a component of the electron transport chain and an enzyme of the Krebs cycle. Complex II deficiencies are seen in OXPHOS genetic disease and found in a type of cancer called paraganglioma.
- Succinate coenzyme Q reductase
- Succinate dehydrogenase
Our Abpromise guarantee covers the use of ab110410 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 6 µg/ml. The antibody cocktail (1.5 mg/mL) should be diluted 250x to a final working concentration of 6 µg/mL for Western blotting.|
All lanes : Complex II WB Antibody Cocktail (ab110410) at 1/250 dilution
Lane 1 : human heart mitochondria at 10 µg
Lane 2 : Immunoprecipitated Complex II at 0.5 µg
The Western blot cocktail ab110410 contains two Complex II subunits SDHA (70 kDa) and SDHB (30 kDa)and a general mitochondrial marker ATP synthase alpha subunit (F1α, 55 kDa). All three are present in the mitochondrial sample (1). The Complex II sample purified by Complex II immunoprecipitation beads (ab109799) shows only Complex II subunits (2).
This product has been referenced in:
- Ma S & Dong Z Melatonin Attenuates Cardiac Reperfusion Stress by Improving OPA1-Related Mitochondrial Fusion in a Yap-Hippo Pathway-Dependent Manner. J Cardiovasc Pharmacol 73:27-39 (2019). Read more (PubMed: 30418242) »
- Hou Y et al. Genetic ablation of TAZ induces HepG2 liver cancer cell apoptosis through activating the CaMKII/MIEF1 signaling pathway. Onco Targets Ther 12:1765-1779 (2019). Read more (PubMed: 30881030) »