Overview

  • Product name

    Anti-Complex III Immunocapture antibody [11A51H12]
  • Description

    Mouse monoclonal [11A51H12] to Complex III Immunocapture
  • Host species

    Mouse
  • Specificity

    100 µg ab109862 can capture at least 10 µg Complex III from 1 mg solubilized bovine heart mitochondria.
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Full length native protein (purified). This information is considered to be commercially sensitive.

  • Positive control

    • Bovine heart and Human mitochondria
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab109862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. PubMed: 22523357
IP Use at 10-1 µg/mg of lysate.

Target

  • Relevance

    Complex III, or ubiquinol cytochrome c oxidoreductase, is a complex of 11 subunits and catalyses electron transfer from reduced ubiquinone or coenzyme Q 10 to cytochrome c. The complex contains 11 different subunits, one of which, cytochrome b, is encoded on mitochondrial DNA, while the remainder subunits are nuclear-encoded. Genetic complex III deficiencies occur but are rare.

Images

  • Staining of Complex III Lane 1: 0.1mg of Bovine mitochondria Lane 2: 0.05mg Bovine mitochondria Lane 3: 0.05mg Human mitochondria Immunoprecipitated using ab109862 at 10 µg/mg lysate.

References

This product has been referenced in:

  • Beutner G  et al. Cyclophilin D regulates the dynamic assembly of mitochondrial ATP synthase into synthasomes. Sci Rep 7:14488 (2017). Read more (PubMed: 29101324) »
  • Beutner G  et al. Initiation of electron transport chain activity in the embryonic heart coincides with the activation of mitochondrial complex 1 and the formation of supercomplexes. PLoS One 9:e113330 (2014). IP ; Mouse . Read more (PubMed: 25427064) »
See all 6 Publications for this product

Customer reviews and Q&As

Answer

Thank you for contacting us. Please click these links to find the suitable antibodies. Type the MS code in Abcam search box to get the Abcam catalogue number. Complex I http://www.mitosciences.com/complex_i.html Complex IV http://www.mitosciences.com/complex_iv.html Complex III http://www.mitosciences.com/complex_iii.html Complex II http://www.mitosciences.com/complex_ii.html We also have Immuno capture kits available https://www.abcam.com/index.html?pageconfig=resource&rid=13917 I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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