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I was wondering for Complex IV Enzyme Activity Dipstick Assay Kit (ab109876), what do you suggest using as a positive control?
Also on page 11 of the protocol, in Table 2: Preparation of Activity Buffer, I was wondering if the volume units for Tube 3 is supposed to be ml and not ul as printed.
Asked on Feb 15 2012
Thank you for your inquiry.
As a positive control, you can use:
Fibroblast extract: 2 - 100ug
Bovine heart mitochondria: 0.03 - 0.6ug
Human muscle extract: 0.5 - 25ug
And yes, you are correct that there is a typo in that table. It should read ml instead of ul. I have alerted our Datasheets Team and they will update that. Thank you for pointing that out to us.
I hope this information helps. Please contact us with any other questions.
Answered on Feb 15 2012