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I am very interested in your dipstick activity assay kit. Especially the complex IV human enzyme dipstick. However, I have a question about this.
Do you have any experience with the accuracy of the complex IV activity in samples which have been frozen?
Specifically I have human periferal blood mononuclear cells dissolved in 70% fetal calf serum, 25% RPMI and 5% DMSO frozen at -80*C and -120*C.
Do you have experience with activity accuracy of any of your other dipstick assay kits on frozen samples?
Asked on May 28 2012
Thank you for contacting us and your interest in our products.
The Complex IV Human Enzyme Activity Dipstick Assay Kit (ab109876)was validated by MitoScienceswith frozen cell pellets (non viable) from patients with SIRF1 and COX10 mutations leading to complex IV deficiency. The attached paper shows the validation data. Your samples should work well with the kitsince the PBMCs were frozen viable.In order to use them with the kit you would first need tothaw them, wash well the FBS with PBS by centrifugation, then solubilize the pellet according to the protocol instructions and measure protein concentration either by 280nm (UV) or by BCA assay before loading on the dipstick.
The paper attached also shows accuracy with the complex I activity dipstick.
I hope this information has been of help. If you have any further questions please do not hesitate to contact us again.
Answered on May 28 2012