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Hi, I'm looking to assay three cell types for mitochondrial protein quantity and activity. I would like to use the dipstick assays by MitoSciences (ab109720, ab109876, ab109722, and ab109877) but really do not need 30 tests of each--only 3. Is there an "assorted" pack I could purchase that just has one (or 5, or 10) of each of the four assays that are offered?
Asked on Aug 02 2012
Thank you for contacting us.
We don't have exactly what you're looking for unfortunately, but we do have MetaPath™ Mito Disease 4-Plex Dipstick Array, ab109879.
The ab109879 is a novel array that allows for the simultaneous quantification of 4 key enzymes whose expression is down-regulated in different mitochondrial diseases: Complex I, Complex IV, PDH and Frataxin. The array is comprised of 4 monoclonal capture antibodies striped onto a dipstick, and the immunocaptured sample is detected using 4 monoclonal detector antibodies that recognize different epitopes on the target enzymes. This array is very rapid (total assay time including sample prep is less than 1 hour) and very sensitive, and as with all Abcam's assays, isolation of mitochondria is not required.
This array is suitable for testing a variety of sample types. Not only is it useful for measuring samples that are deficient in the 4 enzymes due to genetic mutations, it is also useful for measuring changes in the expression of the enzymes due to drug treatments or other manipulations.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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Answered on Aug 02 2012