For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Inquiry: For the protein quantitation of samples in buffer A, which assays besides BCA are compatible with buffer A. will Bradford assay work?
Asked on May 17 2013
Thank you for your inquiry.
I am sorry to confirm that we are not sure what other protein assays will be compatible.
I think Bradford Assay is sensitive to detergents in general and therefore most probably not suitable.
If you like this could be quickly tested by using the extraction buffer alone in the Bradford reagent to see if it changes color.
Another option would be to measure with UV 280 for protein samples. This will also work without any additional material or kits.
I am sorry that I did not have a straight forward answer for you on this occasion and hope this information is nevertheless helpful.
Answered on May 17 2013