Overview

  • Product name
    Complex IV Immunocapture Kit
    See all Complex IV kits
  • Assay type
    Quantitative
  • Species reactivity
    Reacts with: Cow, Human
  • Product overview

    250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 µg, ~50 µg or ~75 µg respectively of Complex IV from heart mitochondria.

    ab109801 Complex IV Immunocapture Kit allows isolation of the cytochrome c oxidase complex from small amounts of tissue. This facilitates subsequent analysis of both assembly state and accrued post-translational modifications of all 13 subunits of the enzyme. The immunoprecipitated Complex IV shows cytochrome c oxidase activity while bound to the beads and this activity is fully sensitive to cyanide. Uses for ab109801 include but are not limited to examining alterations of Complex IV subunits in inherited mitochondrial diseases, Alzheimer's disease, schizophrenia and cancer.

    Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).

  • Tested applications
    Suitable for: IPmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab109801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.

Images

  • Immunoprecipitation - Complex IV Immunocapture Kit (ab109801)
    Immunoprecipitation - Complex IV Immunocapture Kit (ab109801)
    Complex IV immunoprecipitation using antibody ab109863/MS101c crosslinked to protein G-agarose beads as product ab109801

Protocols

References

This product has been referenced in:
  • Oliva CR  et al. Identification of Small Molecule Inhibitors of Human Cytochrome c Oxidase That Target Chemoresistant Glioma Cells. J Biol Chem 291:24188-24199 (2016). IP . Read more (PubMed: 27679486) »
  • Desmurs M  et al. C11orf83, a mitochondrial cardiolipin-binding protein involved in bc1 complex assembly and supercomplex stabilization. Mol Cell Biol 35:1139-56 (2015). Read more (PubMed: 25605331) »

See all 8 Publications for this product

Publishing research using ab109801? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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3 reviews or Q&A

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Votes: Highest First

Customer kindly contacted us to determine if this product might work with zebrafish or goldfish samples.

Thank you for contacting us.

I have been in contact with the lab regarding species reactivity of this product as you have requested. They have tested the paired antibody in zebrafish and this will definitely not work on Zebrafish or goldfi...

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I would like to ask what is the minimum amount of mouse brain tissue required for Immunocapture kit Complex I (as well as complex III, and IV) so as to pull down Complex I. We would need enough material for running an SDS gel.

The MitoSciences laboratory recommends the following: Use 1 mg of brain tissue lysate, eluting into the minimum volume possible. A glycine buffer elution procedure allows the reuse of the beads. If you wish to analyze the product by Weste...

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I would like to ask what is the minimum amount of mouse brain tissue required for Immunocapture kit Complex I (as well as complex III, and IV) so as to pull down Complex I. We would need enough material for running an SDS gel.

Thank you for contacting us. The protocol recommends 300 ug minimum and 5 mg ideally, but these figures refer to isolated mitochondria, and not intact tissue. I have asked the laboratory to send this information, the minimum amount of tissue re...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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