Key features and details
- Assay type: Quantitative
Product nameComplex IV Immunocapture Kit
See all Complex IV kits
Species reactivityReacts with: Cow, Human
ab109801 Complex IV Immunocapture Kit allows isolation of the cytochrome c oxidase complex from small amounts of tissue.
250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 µg, ~50 µg or ~75 µg respectively of Complex IV from heart mitochondria.
This kit facilitates subsequent analysis of both assembly state and accrued post-translational modifications of all 13 subunits of Complex IV. The immunoprecipitated Complex IV shows cytochrome c oxidase activity while bound to the beads and this activity is fully sensitive to cyanide. Uses for ab109801 include but are not limited to examining alterations of Complex IV subunits in inherited mitochondrial diseases, Alzheimer's disease, schizophrenia and cancer.
Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).
Storage instructionsStore at +4°C. Please refer to protocols.
Components 250 µg Monoclonal Complex IV antibody irreversibly crosslinked to protein G-agarose beads 1 x 250µg
- Cytochrome c oxidase
- Cytochrome oxidase
Complex IV immunoprecipitation using antibody ab109863/MS101c crosslinked to protein G-agarose beads as product ab109801
ab109801 has been referenced in 9 publications.
- Morscher RJ et al. Mitochondrial translation requires folate-dependent tRNA methylation. Nature 554:128-132 (2018). PubMed: 29364879
- Oliva CR et al. Identification of Small Molecule Inhibitors of Human Cytochrome c Oxidase That Target Chemoresistant Glioma Cells. J Biol Chem 291:24188-24199 (2016). IP . PubMed: 27679486
- Desmurs M et al. C11orf83, a mitochondrial cardiolipin-binding protein involved in bc1 complex assembly and supercomplex stabilization. Mol Cell Biol 35:1139-56 (2015). PubMed: 25605331
- van den Bosch BJ et al. Defective NDUFA9 as a novel cause of neonatally fatal complex I disease. J Med Genet 49:10-5 (2012). PubMed: 22114105
- Tucker EJ et al. Mutations in MTFMT Underlie a Human Disorder of Formylation Causing Impaired Mitochondrial Translation. Cell Metab 14:428-34 (2011). PubMed: 21907147
- Stiburek L et al. Loss of function of Sco1 and its interaction with cytochrome c oxidase. Am J Physiol Cell Physiol 296:C1218-26 (2009). PubMed: 19295170
- Murray J et al. Small-scale immunopurification of cytochrome c oxidase for a high-throughput multiplexing analysis of enzyme activity and amount. Biotechnol Appl Biochem 48:167-78 (2007). PubMed: 17508937
- Janssen GM et al. The A3243G tRNALeu(UUR) mutation induces mitochondrial dysfunction and variable disease expression without dominant negative acting translational defects in complex IV subunits at UUR codons. Hum Mol Genet 16:2472-81 (2007). PubMed: 17656376
- Walker UA et al. Uridine abrogates the adverse effects of antiretroviral pyrimidine analogues on adipose cell functions. Antivir Ther 11:25-34 (2006). PubMed: 16518957