Product nameComplex IV Immunocapture Kit
See all Complex IV kits
Species reactivityReacts with: Cow, Human
ab109801 Complex IV Immunocapture Kit allows isolation of the cytochrome c oxidase complex from small amounts of tissue.
250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 µg, ~50 µg or ~75 µg respectively of Complex IV from heart mitochondria.
This kit facilitates subsequent analysis of both assembly state and accrued post-translational modifications of all 13 subunits of Complex IV. The immunoprecipitated Complex IV shows cytochrome c oxidase activity while bound to the beads and this activity is fully sensitive to cyanide. Uses for ab109801 include but are not limited to examining alterations of Complex IV subunits in inherited mitochondrial diseases, Alzheimer's disease, schizophrenia and cancer.
Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).
Storage instructionsStore at +4°C. Please refer to protocols.
Components 250 µg ab109801 - monoclonal Complex IV antibody irreversibly crosslinked to protein G-agarose beads 1 x 250µg
- Cytochrome c oxidase
- Cytochrome oxidase
Complex IV immunoprecipitation using antibody ab109863/MS101c crosslinked to protein G-agarose beads as product ab109801
This product has been referenced in:
- Morscher RJ et al. Mitochondrial translation requires folate-dependent tRNA methylation. Nature 554:128-132 (2018). Read more (PubMed: 29364879) »
- Oliva CR et al. Identification of Small Molecule Inhibitors of Human Cytochrome c Oxidase That Target Chemoresistant Glioma Cells. J Biol Chem 291:24188-24199 (2016). IP . Read more (PubMed: 27679486) »