Complex IV Immunocapture Kit (ab109801)

Thank you for contacting us.

I have been in contact with the lab regarding species reactivity of this product as you have requested. They have tested the paired antibody in zebrafish and this will definitely not work on Zebrafish or goldfish.

While not IP or immunocapture some of the mitosciences antibodies were used in a mitochondrial zebrafish project:

http://www.ncbi.nlm.nih.gov/pubmed?term=17761683

Should you wish to test any Abcam products in an untested species or application we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount equal to the value of the product tested. The terms and conditions of this offer can be found here: https://www.abcam.com/AbTrial

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

The MitoSciences laboratory recommends the following: Use 1 mg of brain tissue lysate, eluting into the minimum volume possible. A glycine buffer elution procedure allows the reuse of the beads. If you wish to analyze the product by Western blot, we can scale back the amount of material required. So using whole tissue I think you can be very confident using 1 mg of tissue or less. However, be aware of possible mouse capture antibody elution from the beads into the sample when blotting. Ways to resolve this problem: (1) Do not include reducing agent in sample during electrophoresis. This should maintain eluted antibody at 200 kDa during electrophoresis. (2) Western blotting – Blot with rabbit antibodies or use a secondary detection system that recognizes only the native mouse antibody being used to Western blot.

Thank you for contacting us. The protocol recommends 300 ug minimum and 5 mg ideally, but these figures refer to isolated mitochondria, and not intact tissue. I have asked the laboratory to send this information, the minimum amount of tissue required, and I will forward this when I receive it. If you are interested in isolating mitochondria, which will make the immunocapture more efficient and the yield more pure, I recommend considering the mitochondria isolation kits, ab110168 and ab110169.