Complex IV Rodent Enzyme Activity Dipstick Assay Kit (ab109878)


  • Product name
    Complex IV Rodent Enzyme Activity Dipstick Assay Kit
    See all Complex IV kits
  • Sample type
    Cell culture extracts, Tissue
  • Assay type
    Sandwich (quantitative)
  • Species reactivity
    Reacts with: Mouse, Rat
  • Product overview

    ab109878(MS432) contains 30 or 90 dipsticks and necessary components to quantify the activity of the cytochrome c oxidase enzyme complex from mouse and rat samples. The kit includes sufficient materials to generate a standard curve and evaluate several unknown samples.

    Using an antibody immobilized on the dipstick that specifically recognizes COX, the enzyme complex is immno-captured (i.e immuno-precipitated in active form) and enzyme activity is determined directly on the dipstick. Using a traditional method for determining COX enzyme activity by histo-chemical methods and in-gel activity assays, Di-amino benzidinetetrachloride (DAB) serves as the reporter of COX activity. The greater the signal of precipitated DAB, the greater the amount of COX enzyme complex. In addition, the reaction is cyanide sensitive. The signal intensity is best measured by a dipstick reader or may be analyzed by another imaging system.

  • Notes

    Store dipsticks at room temperature in their provided container and out of direct sunlight.

    High humidity conditions should be avoided.

    Store Buffer A, B, and C at 4°C or at -20°C for long term storage.

    Store Tubes 1 and 2 at -20°C or at -80°C for long term storage; they can also be aliquoted upon receipt to prevent freeze/thaw cycles.

    Tube 3 can be stored at room temperature.

  • Tested applications
    Suitable for: Functional Studiesmore details
  • Platform



Our Abpromise guarantee covers the use of ab109878 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.


  • Abcam's enzyme activity assays apply a novel approach, whereby target enzymes are first immunocaptured from tissue or cell samples before subsequent functional analysis. Dipstick ELISA Kits extend this concept by utilizing the well-established lateral flow concept, wherein capture antibodies are striped onto nitrocellulose membrane and a wicking pad draws the sample through the antibody bands. All of our ELISA kits utilize highly validated monoclonal antibodies and proprietary buffers, which are able to capture even very large enzyme complexes in their fully-intact, functionally-active states.
  • Figure 1. An example using ab109878 to quantify Complex IV enzyme activity in mouse protein extracts Developed dipsticks from a 1:2 dilution series using a positive control sample and the associated standard curve. Starting material was 100 µg of protein extract.
  • Figure 2. An example using ab109878 to quantify Complex IV enzyme activity in mouse protein extracts Based on the standard curve, 50 µg of protein extract were loaded onto a dipstick for each sample. The figure shows three developed dipsticks from unknown samples (1-3). The analysis of the signal intensity and interpolation from the standard curve showed that the unknown samples have between 23-87% of normal Complex IV activity levels.



This product has been referenced in:
  • Chavan H  et al. Arsenite Effects on Mitochondrial Bioenergetics in Human and Mouse Primary Hepatocytes Follow a Nonlinear Dose Response. Oxid Med Cell Longev 2017:9251303 (2017). Read more (PubMed: 28163822) »
  • Vafai SB  et al. Natural Product Screening Reveals Naphthoquinone Complex I Bypass Factors. PLoS One 11:e0162686 (2016). Functional Studies . Read more (PubMed: 27622560) »

See all 4 Publications for this product

Customer reviews and Q&As

Thank you or your inquiry.

Please find below the answer of one of our senior scientists to your question:

"Iam assuming that the customer refers to high ATP/ADP ratios reducing the activity of Cytochrome c Oxidase, making it th...

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I can confirm that Dipsticks could be scanned with a regular document scanner and then signal intensity of the image could be analyzed with Image J software (which can be downloaded for Free).

The other o...

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The buffers for each kit are optimized for use with the particular assay. The name 'A', 'B', etc. is by convention and not representative of similar components.

I hope this information is helpful t...

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Yes, both kits work with frozen mouse tissue samples.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us.  1. The detergent used in this kit is not efficient at extracting nuclear samples.  Most of the nuclear proteins will be in the pellet obtained after the centrifugation step during the sample preparation (as explained i...

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Thank you for contacting us. Please find further information below about the use of protease inhibitors: When should protease inhibitors be added?   Protease inhibitors should be added during the extraction procedure.  Which p...

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