Product nameComplex IV Rodent Immunocapture Kit
See all Complex IV kits
Species reactivityReacts with: Mouse, Rat
Complex IV Rodent Immunocapture Kit ab109860 allows for isolation of the cytochrome c oxidase complex from small amounts of tissue.
250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 µg, ~50 µg or ~75 µg respectively of Complex IV from heart mitochondria.
This kit facilitates the analysis of both assembly state and accrued post-translational modifications of all 13 subunits of the cytochrome c oxidase complex. The immunoprecipitated Complex IV shows cytochrome c oxidase activity while bound to the beads and this activity is fully sensitive to cyanide. Uses for ab109860 include but are not limited to examining alterations of Complex IV subunits in inherited mitochondrial diseases, Alzheimer's disease, schizophrenia and cancer.
Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).
Storage instructionsStore at +4°C. Please refer to protocols.
Components 250 µg monoclonal antibody irreversibly crosslinked to protein G-agarose beads 1 x 250µg
- Cytochrome c oxidase
- Cytochrome oxidase
ab109860 has been referenced in 1 publication.
- Strub GM et al. Sphingosine-1-phosphate produced by sphingosine kinase 2 in mitochondria interacts with prohibitin 2 to regulate complex IV assembly and respiration. FASEB J 25:600-12 (2011). PubMed: 20959514