Compound 55, DOT1L Inhibitor, DOT1L inhibitor (ab145525)

Overview

  • Product name

    Compound 55, DOT1L Inhibitor, DOT1L inhibitor
  • Description

    Highly potent, selective DOT1L inhibitor
  • Biological description

    Highly potent, selective DOT1L inhibitor (Ki = 0.46 nM). Shows antiproliferative effects (EC50 values are 4.4, 8.1 and 10 μM for MV4-11, THP1 and NB4 leukemia cells, respectively). Binds to the DOT1L:nucleosome complex (Kd = 66 nM). Competes with SAM/SAH, but not the substrate nucleosome, on binding to DOT1L.
  • Purity

    > 98%
  • CAS Number

    1381761-52-9
  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    5'-Deoxy-5'-[[3-[[[[4-(1,1-dimethylethyl)phenyl]amino]carbonyl]amino]propyl](1-methylethyl)amino]adenosine hydrochloride
  • Molecular weight

    577.13
  • Molecular formula

    C27H40N8O4.HCl
  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in water to 50 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    Cl.CC(C)(C)c1ccc(cc1)NC(=O)NCCCN(C[C@H]4O[C@@H](n3cnc2c(N)ncnc23)C(O)[C@H]4O)C(C)C
  • Source

    Synthetic

References

This product has been referenced in:

  • Anglin JL  et al. Synthesis and structure-activity relationship investigation of adenosine-containing inhibitors of histone methyltransferase DOT1L. J Med Chem 55:8066-74 (2012). Read more (PubMed: 22924785) »
  • Daigle SR  et al. Selective killing of mixed lineage leukemia cells by a potent small-molecule DOT1L inhibitor. Cancer Cell 20:53-65 (2011). Read more (PubMed: 21741596) »
  • Yao Y  et al. Selective inhibitors of histone methyltransferase DOT1L: design, synthesis, and crystallographic studies. J Am Chem Soc 133:16746-9 (2011). Read more (PubMed: 21936531) »

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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