Overview

  • Product name

  • Description

    Rabbit polyclonal to COMT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 221-271 of Human COMT (NP_000745.1).

  • Positive control

    • Whole cell lysate from HeLa, 293T and Jurkat cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab129504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 30 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.
  • Tissue specificity

    Brain, liver, placenta, lymphocytes and erythrocytes.
  • Sequence similarities

    Belongs to the mammalian catechol-O-methyltransferase family.
  • Post-translational
    modifications

    The N-terminus is blocked.
  • Cellular localization

    Cytoplasm and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Catechol O methyltransferase antibody
    • Catechol O-methyltransferase antibody
    • COMT antibody
    • COMT_HUMAN antibody
    • EC 2.1.1.6 antibody
    see all

Images

  • All lanes : Anti-COMT antibody (ab129504) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from 293T cells at 50 µg
    Lane 4 : Whole cell lysate from Jurkat cells at 50 µg

    Developed using the ECL technique.

    Predicted band size: 30 kDa


    Exposure time: 3 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling COMT with ab129504 at 1/1000 (1µg/ml). Detection: DAB.
  • ab129504 at 1 µg/ml staining COMT by WB, following immunoprecipitation of whole cell lysate from HeLa cells.
    Lane 1; IP using ab129504 at 6 µg/mg lysate.
    Lane 2; IP using control IgG.
    1 mg of lysate was used for IP and 20% of IP was loaded. Detection utilised Chemiluminescence with a 10 second exposure.

References

ab129504 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: High and Low pH Citrate buffer
Permeabilization
Yes - 0.1% Triton-X-100
Specification
Brain
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Z-fix

Abcam user community

Verified customer

Submitted Aug 05 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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