Anti-COMT antibody (ab129504)
Key features and details
- Rabbit polyclonal to COMT
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-COMT antibody
See all COMT primary antibodies -
Description
Rabbit polyclonal to COMT -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 221-271 of Human COMT (NP_000745.1).
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Positive control
- Whole cell lysate from HeLa, 293T and Jurkat cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (1) |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 30 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| Notes |
|---|
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 30 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol. -
Tissue specificity
Brain, liver, placenta, lymphocytes and erythrocytes. -
Sequence similarities
Belongs to the mammalian catechol-O-methyltransferase family. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Cytoplasm and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1312 Human
- Omim: 116790 Human
- SwissProt: P21964 Human
- Unigene: 370408 Human
- Unigene: 704514 Human
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Alternative names
- Catechol O methyltransferase antibody
- Catechol O-methyltransferase antibody
- COMT antibody
see all
Images
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Western blot - Anti-COMT antibody (ab129504)All lanes : Anti-COMT antibody (ab129504) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from 293T cells at 50 µg
Lane 4 : Whole cell lysate from Jurkat cells at 50 µg
Developed using the ECL technique.
Predicted band size: 30 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COMT antibody (ab129504)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling COMT with ab129504 at 1/1000 (1µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-COMT antibody (ab129504)ab129504 at 1 µg/ml staining COMT by WB, following immunoprecipitation of whole cell lysate from HeLa cells.
Lane 1; IP using ab129504 at 6 µg/mg lysate.
Lane 2; IP using control IgG.
1 mg of lysate was used for IP and 20% of IP was loaded. Detection utilised Chemiluminescence with a 10 second exposure.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab129504 has not yet been referenced specifically in any publications.