Anti-COMT antibody (ab226938)
Key features and details
- Rabbit polyclonal to COMT
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-COMT antibody
See all COMT primary antibodies -
Description
Rabbit polyclonal to COMT -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rhesus monkey
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Immunogen
Recombinant fragment within Human COMT (internal sequence). The exact sequence is proprietary.
Database link: P21964 -
Positive control
- WB: MOLT-4 whole cell lysate; Mouse spleen lysate. IHC-P: Human colon carcinoma tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab226938 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 30 kDa. | |
| IHC-P | 1/100 - 1/1000. |
Target
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Function
Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol. -
Tissue specificity
Brain, liver, placenta, lymphocytes and erythrocytes. -
Sequence similarities
Belongs to the mammalian catechol-O-methyltransferase family. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Cytoplasm and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1312 Human
- Entrez Gene: 12846 Mouse
- Entrez Gene: 712548 Rhesus monkey
- Omim: 116790 Human
- SwissProt: P21964 Human
- SwissProt: O88587 Mouse
- Unigene: 370408 Human
- Unigene: 704514 Human
see all -
Alternative names
- Catechol O methyltransferase antibody
- Catechol O-methyltransferase antibody
- COMT antibody
see all
Images
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Western blot - Anti-COMT antibody (ab226938)Anti-COMT antibody (ab226938) at 1/500 dilution + Mouse spleen lysate at 50 µg
Predicted band size: 30 kDa12 % SDS-PAGE gel.
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Western blot - Anti-COMT antibody (ab226938)Anti-COMT antibody (ab226938) at 1/1000 dilution + MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate at 30 µg
Predicted band size: 30 kDa12 % SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COMT antibody (ab226938)Paraffin-embedded human colon carcinoma tissue stained for COMT with ab226938 at 1/250 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab226938 has not yet been referenced specifically in any publications.
