Recombinant Anti-COMT antibody [EPR6490] (ab126618)
![Western blot - Anti-COMT antibody [EPR6490] (ab126618)](https://www.abcam.com/ps/products/126/ab126618/Images/ab126618-388720-anti-comt-antibody-epr6490-western-blot-hek293t-lysates.jpg "Western blot - Anti-COMT antibody [EPR6490] (ab126618)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6490] to COMT
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Overview
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Product name
Anti-COMT antibody [EPR6490]
See all COMT primary antibodies -
Description
Rabbit monoclonal [EPR6490] to COMT -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide corresponding to Human COMT. Human COMT is a soluble isoform.
Database link: P21964 -
Positive control
- WB: HEK-293T, HAP1, A431, MOLT4 and MCF7 cell lysates; Human fetal liver lysate. IHC-P: Human liver and kidney tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6490 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126618 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 24-30 kDa (predicted molecular weight: 30 kDa).
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 24-30 kDa (predicted molecular weight: 30 kDa). |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol. -
Tissue specificity
Brain, liver, placenta, lymphocytes and erythrocytes. -
Sequence similarities
Belongs to the mammalian catechol-O-methyltransferase family. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Cytoplasm and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1312 Human
- Entrez Gene: 12846 Mouse
- Entrez Gene: 24267 Rat
- Omim: 116790 Human
- SwissProt: P21964 Human
- SwissProt: O88587 Mouse
- SwissProt: P22734 Rat
- Unigene: 370408 Human
see all -
Alternative names
- Catechol O methyltransferase antibody
- Catechol O-methyltransferase antibody
- COMT antibody
see all
Images
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Western blot - Anti-COMT antibody [EPR6490] (ab126618)All lanes : Anti-COMT antibody [EPR6490] (ab126618) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : COMT knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 24-28 kDa why is the actual band size different from the predicted?Lanes 1-2: Merged signal (red and green). Green - ab126618 observed at 24-28 kDa. Red - loading control ab8245 observed at 37 kDa.
ab126618 Anti-COMT antibody [EPR6490] was shown to specifically react with COMT in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266537 (knockout cell lysate ab257396) was used. Wild-type and COMT knockout samples were subjected to SDS-PAGE. ab126618 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COMT antibody [EPR6490] (ab126618)](https://www.abcam.com/ps/products/126/ab126618/Images/ab126618-140606-anti-comt-antibody-epr6490-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COMT antibody [EPR6490] (ab126618)ab126618, at 1/50 dilution, staining COMT in Formalin-fixed, Paraffin-embedded Human liver tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-COMT antibody [EPR6490] (ab126618)](https://www.abcam.com/ps/products/126/ab126618/Images/ab126618-271528-anti-comt-antibody-epr6490-western-blot.jpg)
Western blot - Anti-COMT antibody [EPR6490] (ab126618)All lanes : Anti-COMT antibody [EPR6490] (ab126618) at 1/1000 dilution
Lane 1 : Wild type HAP1 whole cell lysate at 40 µg
Lane 2 : COMT knockout HAP1 whole cell lysate at 40 µg
Lane 3 : A431 whole cell lysate at 20 µg
Lane 4 : MCF7 whole cell lysate at 20 µg
Predicted band size: 30 kDaLanes 1 - 4: Merged signal (red and green). Green - ab126618 observed at 24/28 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab126618 was shown to specifically react with COMT when COMT knockout samples were used. Wild-type and COMT knockout samples were subjected to SDS-PAGE. Samples were incubated with ab126618 and ab8245 (Mouse anti-GAPDH loading control) overnight at 4°C at 1/1000 and 1/10000 dilutions respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-COMT antibody [EPR6490] (ab126618)](https://www.abcam.com/ps/products/126/ab126618/Images/ab126618-140608-anti-comt-antibody-epr6490-western-blot.jpg)
Western blot - Anti-COMT antibody [EPR6490] (ab126618)All lanes : Anti-COMT antibody [EPR6490] (ab126618) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : A431 cell lysate
Lane 3 : MOLT4 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-rabbit HRP at 1/2000 dilution
Predicted band size: 30 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COMT antibody [EPR6490] (ab126618)](https://www.abcam.com/ps/products/126/ab126618/Images/ab126618-140607-anti-comt-antibody-epr6490-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COMT antibody [EPR6490] (ab126618)ab126618, at 1/50 dilution, staining COMT in Formalin-fixed, Paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-COMT antibody [EPR6490] (ab126618)](https://www.abcam.com/ps/products/126/ab126618/Images/ab126618-6-benefits-of-recombinant-antibodies.png)
Anti-COMT antibody [EPR6490] (ab126618)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab126618 has been referenced in 5 publications.
- Winner BM et al. Metabolism of Dopamine in Nucleus Accumbens Astrocytes Is Preserved in Aged Mice Exposed to MPTP. Front Aging Neurosci 9:410 (2017). PubMed: 29311899
- Cooper TK et al. Coati Bodies: Cytoplasmic Hyaline Globules in the Ganglionic Neurons of Aging Captive Coatis. Vet Pathol 54:851-854 (2017). PubMed: 28578627
- Doan KV et al. FoxO1 in dopaminergic neurons regulates energy homeostasis and targets tyrosine hydroxylase. Nat Commun 7:12733 (2016). PubMed: 27681312
- Stefanovic B et al. Melatonin mediated antidepressant-like effect in the hippocampus of chronic stress-induced depression rats: Regulating vesicular monoamine transporter 2 and monoamine oxidase A levels. Eur Neuropsychopharmacol 26:1629-37 (2016). WB ; Rat . PubMed: 27499503
- Wakuda T et al. Perinatal asphyxia alters neuregulin-1 and COMT gene expression in the medial prefrontal cortex in rats. Prog Neuropsychopharmacol Biol Psychiatry 56:149-54 (2015). PubMed: 25194460